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A3643

Sigma-Aldrich

Agarose

Type IV, Special High EEO

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Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

biological source

algae (marine)

Quality Level

type

Type IV

form

powder

technique(s)

electrophoresis: suitable

EEO

≥0.3

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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1 of 4

This Item
A5304A2790A2929
Agarose Type IV, Special High EEO

Sigma-Aldrich

A3643

Agarose

Agarose Special High EEO

Sigma-Aldrich

A5304

Agarose

Agarose Wide range, for molecular biology

Sigma-Aldrich

A2790

Agarose

Agarose For pulsed field electrophoresis running gel

Sigma-Aldrich

A2929

Agarose

form

powder

form

powder

form

powder

form

powder

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable, immunoelectrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

EEO

≥0.3

EEO

≤0.40

EEO

≤0.15

EEO

≤0.10

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

type

Type IV

type

-

type

-

type

-

Application

Agarose has been used in the electrophoresis of RNA for northern blot. It has also been used in coating microscope slides for counter immunoelectrophoresis.

Quality

Characterized by lower sulfate content and lower non-specific protein binding capacity than Type III. Trailing and smearing of proteins due to non-specific protein binding is minimized.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Recommended products

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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