A4018

Sigma-Aldrich

Agarose, low gelling temperature

Synonym(s):
2-Hydroxyethyl agarose
CAS Number:
MDL number:
NACRES:
NA.21

Quality Level

biological source

algae (marine)

application(s)

cell culture | mammalian: suitable

EEO

≤0.10

transition temp

congealing temperature 26-30 °C

gel strength

≥200 g/cm2 (1% gel)

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General description

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible and possesses variable mechanical and diffusion properties. Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically. Low melting or low gelling temperature agarose is produced by hydroxyethylation of agarose. It is usually used for the isolation of separated DNA fragments. DNA fragments of the equal size will take longer time to move through a low melting agarose gel compared to a standard agarose gel.

Application

Gels exhibit excellent clarity and are particularly useful for the preparation of media containing heat-labile materials. Recommended for preparation of agarose beads.
Agarose, low gelling temperature has been used:
  • to immobilize zebra fish embryos during in vivo imaging experiments
  • as a constituent of defined medium to culture bovine nucleus pulposus (NP) cells
  • as a hydrogel, a scaffolding material for dental pulp regeneration
  • in the form of pellets to determine the surface pH and conductivity of an acrylic emulsion film

Packaging

1, 5, 10, 25, 50, 100 g in poly bottle

Quality

A low gelling temperature derivative with unique gelling properties.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

In vivo imaging of zebrafish retina
Williams PR, et al.
Cold Spring Harbor Protocols, 2013(1) (2013)
Nucleus pulposus cells synthesize a functional extracellular matrix and respond to inflammatory cytokine challenge following long term agarose culture
Smith LJ
European Cells & Materials, 22, 291-291 (2011)
Methods in Enzymology, 135, 223-223 (1987)
Xiaoquan Yang et al.
Optics express, 25(7), 7349-7357 (2017-04-07)
An optical-resolution photoacoustic microscope (OR-PAM) with capability of fast axial-scanning was developed by using a tunable acoustic gradient (TAG) lens. The TAG lens was designed to continuously changing the focal plane of OR-PAM by modulating its refractive power with fast-changing...
Testing the Waters: New Technical Applications for the Cleaning of Acrylic Paint Films and Paper Supports
The Book and Paper Group Annual, 32, 43-51 (2013)
Articles
The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.
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Protocols
The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.
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