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A4718

Sigma-Aldrich

Agarose

for molecular biology

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Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

biological source

algae (marine)

Quality Level

grade

for molecular biology

form

powder

technique(s)

electrophoresis: suitable

EEO

≤0.05

mp

≤75 °C (3% gel)

transition temp

gel point ≤35 °C (3% gel)

foreign activity

DNase, RNase, NICKase, none detected

storage temp.

room temp

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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This Item
A6013A6877A2929
Agarose for molecular biology

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A4718

Agarose

Agarose Type I, low EEO

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A6013

Agarose

Agarose Medium EEO, for molecular biology

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Agarose For pulsed field electrophoresis running gel

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Agarose

grade

for molecular biology

grade

-

grade

for molecular biology

grade

-

form

powder

form

powder

form

powder

form

powder

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

electrophoresis: suitable

EEO

≤0.05

EEO

0.09-0.13

EEO

0.16-0.19

EEO

≤0.10

transition temp

gel point ≤35 °C (3% gel)

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

transition temp

congealing temperature 34.5-37.5 °C (1.5% gel)

transition temp

gel point 38-43 °C (10% gel)

General description

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible and possesses variable mechanical and diffusion properties. Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically.

Application

Agarose has been used in the preparation of microgel slides employed in lysis and electrophoresis. It has also been used in the gel electrophoresis to separate restriction enzyme digested products in catechol-O-methyltransferase (COMT) genotyping
Ideal for the separation of small DNA fragments (e.g., PCR products) differing in size by as little as 2% and compares to the resolution of DNA in polyacrylamide gels. High resolution agarose is also ideal for resolving amplification fragment length polymorphisms (AmpFLP), short tandem repeats (STR) and tri- and tetranucleotide repeats.
Low ethidium bromide and SYBR Green background staining

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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