A5431

Noble agar

• Synonym(s): Agar, Agar-agar, Gum agar
• Linear Formula: (C₁₂H₁₈O₉)_n
• CAS Number: 9002-18-0
• EC Number: 232-658-1
• MDL number: MFCD00081288
• UNSPSC Code: 50405901
• NACRES: NA.75

Properties

• form: powder
• Quality Level: 200
• application(s): food and beverages
• SMILES string: O1[C@@H]([C@@H]([C@@H]([C@H](C1OC2[C@H]3OCC2OC([C@H]3O)C)O)OC)O)CO
• InChI: 1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
• InChI key: GYYDPBCUIJTIBM-DYOGSRDZSA-N

Description

General description

Noble agar, also known as agar or agar-agar, is a gelling hydrocolloid extracted from sea weeds such as Gelidium and Gracilaria.[1][2] It′s structure contains repeating units of D-galactose and 3,6-anhydro-L-galactose, with a few differences, in addition to a low ester sulphate residue.[1] Agarose and agaropectin are the key components of agar.[3] Noble agar is majorly used as both human and animal food supplement. In addition, it is also used in industries for the preparation of solid microbiological media.[2]

Application

Noble agar has been used as a component of growth medium for High grade serous ovarian cancer (HGSOC) cell lines for soft agar colony assay.[4] It has also been used as a media component to culture anaerobic bacteria, Bacteroides thetaitaomicron[5]

Noble agar has been used to make agar gel plates that acts as a standard solid support material:

• to culture tumor spheroids[6][7]
• in lysoplate assay method of Osserman & Lawlor to measure lysozyme concentration in albumen or plasma[8][9]
• as BG11 media component to culture Scenedesmus obtusiusculus strains[10]

Originally designed for use in Noble′s cyanide citrate agar.

Preparation Note

Further purified from bacteriological agar with careful specific washings.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• ppe: Eyeshields, Gloves, type N95 (US)