A6877

Sigma-Aldrich

Agarose

Medium EEO, for molecular biology

CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

biological source

algae (marine)

grade

for molecular biology

type

Type II

form

powder

impurities

≤10% moisture content

EEO

0.16-0.19

transition temp

congealing temperature 34.5-37.5 °C (1.5% gel)

gel strength

≥1000 g/cm2 (1.0% gel)

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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General description

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible, possesses variable mechanical and diffusion properties.

Application

Agarose has been used:
  • in gel electrophoresis
  • in the preparation of 2x agarose solution used along with 2x supplemented-MEM media as an overlay medium in plaque assay
  • in an immunodiffusion test for Paracoccidioidomycosis

Packaging

5, 10, 25, 50, 100, 250, 500 g in poly bottle
1 kg in poly bottle

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests.
De Camargo Z, et al.
Journal of Clinical Microbiology, 26(10), 2147-2151 (1988)
A 16S rRNA-based DNA probe and PCR method specific for Listeria ivanovii
Wang R, et al.
FEMS Microbiology Letters, 106(1), 85-92 (1993)
A feline kidney cell line-based plaque assay for feline calicivirus, a surrogate for Norwalk virus
Bidawid S, et al.
Journal of Virological Methods, 107(2), 163-167 (2003)
Gregory Gegembauer et al.
PLoS neglected tropical diseases, 8(7), e2986-e2986 (2014-07-18)
Paracoccidioides lutzii is a new agent of paracoccidioidomycosis (PCM) and has its epicenter localized to the Central-West region of Brazil. Serological diagnosis of PCM caused by P. lutzii has not been established. This study aimed to develop new antigenic preparations...
A new method of screening of the silkworm egg with multiprimer PCR
Meena R, et al.
Journal of Invertebrate Pathology, 82(3), 148-151 (2003)

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