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A7002

Sigma-Aldrich

Agar

ash 2.0-4.5%

Synonym(s):
Agar-agar, Gum agar
Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
eCl@ss:
42040102
NACRES:
NB.22

Quality Level

biological source

algae (Rhodophyceae)

form

flakes
powder or crystals

application(s)

tissue culture: suitable

ash

2.0-4.5%

gel strength

600-1200 g/cm2 (1.5%, 20 oC)

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General description

Agar is a polysaccharide consisting of β- D-galactopyranosyl and 3,6-anhydro-α-L- galactopyranosyl units. Depending on the source agar exists as various substituted forms such as methyl and sulphate ether. It is a linear galactan polysaccharide isolated from the cell wall of Rhodophyceae class of seaweeds.

Application

Agar has been used:
  • as a component of solid N6 complete medium
  • in modified Murashige and Skoog (MS) medium for culturing germinating lentil and faba bean seeds and in the regeneration of transgenic cauliflower and broccoli explants
  • as a component of agar mixture to simulate a vascular phantom in carotid vessel

Packaging

100, 250, 500 g in poly bottle
1 kg in poly bottle
5 kg in poly drum

Biochem/physiol Actions

Agar exists in left-handed helical conformation and aggregation of hexagonal fibers leads to gel formation. The hydrophobicity of the disaccharides determines its gelling property and solubility. Agar is a major component of the microbial solid medium. It has applications in tissue engineering, pharmaceutics and in cell encapsulation.

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

A Method for Radioactive labelling of Hebeloma cylindrosporum to study plant-fungus interactions
Becquer A, et al.
Bio-protocol, 7(20), 550-554 (2017)
Chemical structure and physico-chemical properties of agar
International Workshop on Gelidium, 21(11), 137-148 (1991)
Successful preparation and characterization of biotechnological grade agarose from indigenous Gelidium amansii of Taiwan
Wang TP, et al.
Process Biochemistry (Oxford, United Kingdom), 47(3), 550-554 (2012)
Plant growth regulators improve in vitro flowering and rapid generation advancement in lentil and faba bean
Mobini SH, et al.
In Vitro Cellular & Developmental Biology, 51(1), 71-79 (2015)
Analysis of carotid lumen surface morphology using three-dimensional ultrasound imaging
Chiu B, et al.
Physics in Medicine and Biology, 54(5), 1149-1149 (2009)

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