A7002

Sigma-Aldrich

Agar

ash 2.0-4.5%

Synonym(s):
Agar-agar, Gum agar
Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
eCl@ss:
42040102
NACRES:
NB.22

Quality Level

biological source

algae (Rhodophyceae)

form

flakes
powder or crystals

application(s)

tissue culture: suitable

ash

2.0-4.5%

gel strength

600-1200 g/cm2 (1.5%, 20 oC)

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General description

Agar is a polysaccharide consisting of β- D-galactopyranosyl and 3,6-anhydro-α-L- galactopyranosyl units. Depending on the source agar exists as various substituted forms such as methyl and sulphate ether. It is a linear galactan polysaccharide isolated from the cell wall of Rhodophyceae class of seaweeds.

Application

Agar has been used:
  • as a component of solid N6 complete medium
  • in modified Murashige and Skoog (MS) medium for culturing germinating lentil and faba bean seeds and in the regeneration of transgenic cauliflower and broccoli explants
  • as a component of agar mixture to simulate a vascular phantom in carotid vessel

Packaging

100, 250, 500 g in poly bottle
1 kg in poly bottle
5 kg in poly drum

Biochem/physiol Actions

Agar exists in left-handed helical conformation and aggregation of hexagonal fibers leads to gel formation. The hydrophobicity of the disaccharides determines its gelling property and solubility. Agar is a major component of the microbial solid medium. It has applications in tissue engineering, pharmaceutics and in cell encapsulation.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

Successful preparation and characterization of biotechnological grade agarose from indigenous Gelidium amansii of Taiwan
Wang TP, et al.
Process Biochemistry (Oxford, United Kingdom), 47(3), 550-554 (2012)
Plant growth regulators improve in vitro flowering and rapid generation advancement in lentil and faba bean
Mobini SH, et al.
In Vitro Cellular & Developmental Biology, 51(1), 71-79 (2015)
Recovery of phenotypically normal transgenic plants of Brassica oleracea upon Agrobacterium rhizogenes-mediated co-transformation and selection of transformed hairy roots by GUS assay
Puddephat IJ, et al.
Molecular breeding : new strategies in plant improvement, 7(3), 229-242 (2001)
Analysis of carotid lumen surface morphology using three-dimensional ultrasound imaging
Chiu B, et al.
Physics in Medicine and Biology, 54(5), 1149-1149 (2009)
Niranjan Khadka et al.
Neuromodulation : journal of the International Neuromodulation Society, 21(4), 334-339 (2017-01-24)
To assess if transcranial direct current stimulation (tDCS) produces a temperature change at the skin surface, if any change is stimulation polarity (anode or cathode) specific, and the contribution of passive heating (joule heat) or blood flow on such change....

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