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MilliporeSigma

A7174

Sigma-Aldrich

Agarose

High Gelling Temperature

Synonym(s):

3,6-Anhydro-α-L-galacto-β-D-galactan

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10 G
$167.40
25 G
$367.00

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41105317
PubChem Substance ID:
NACRES:
NA.25

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$167.40

List Price$186.00Save 10%
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In StockDetails


Ships Every 4 weeks
Technical Service
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Quality Level

type

Type VI-A

form

powder

impurities

≤10% water

ash

≤0.60%

EEO

≤0.14

transition temp

gel point 41 °C ±1.5 °C (1.5% gel)

gel strength

≥900 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.20%

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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A5030A2576A2929
Agarose High Gelling Temperature

A7174

Agarose

Agarose Ultra-low Gelling Temperature

A5030

Agarose

Agarose Ultra-low Gelling Temperature, molecular biology grade

A2576

Agarose

Agarose For pulsed field electrophoresis running gel

A2929

Agarose

form

powder

form

powder

form

powder

form

powder

EEO

≤0.14

EEO

≤0.05

EEO

≤0.12

EEO

≤0.10

gel strength

≥900 g/cm2 (1% gel)

gel strength

-

gel strength

≥300 g/cm2 (1.5% gel)

gel strength

≥2000 g/cm2

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

impurities

≤10% water

impurities

-

impurities

≤7% water

impurities

≤10% water

anion traces

sulfate (SO42-): ≤0.20%

anion traces

-

anion traces

sulfate (SO42-): ≤0.14%

anion traces

sulfate (SO42-): ≤0.20%

Application


  • Quality and applicability of cadaveric donor eyes for molecular biology research: An Indian experience.: This study discusses the use of agarose in preparing donor eyes for molecular biology research, highlighting its effectiveness in preserving samples for high-quality genetic analysis (Kaur et al., 2024).

  • Immunocytochemistry on frozen-embedded cell block for the diagnosis of hematolymphoid cytology specimen: a straightforward alternative to the conventional cell block.: Agarose is used to enhance the immunocytochemistry process, providing a more efficient and clear diagnostic method for hematolymphoid diseases (Choi et al., 2024).

  • Low-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics.: Demonstrates the use of low-melting point agarose as a versatile medium for advanced imaging and proteomic analysis, contributing significantly to analytical biochemistry (McDonnell et al., 2023).

  • Autocrine regulation of tumor cell repopulation by Hsp70-HMGB1 alarmin complex.: Agarose is utilized in the study of cellular mechanisms for cancer research, underscoring its importance in experimental setups requiring precise biological simulations (Guzhova et al., 2023).

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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