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A7174

Sigma-Aldrich

Agarose

High Gelling Temperature

Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

type

Type VI-A

form

powder

impurities

≤10% water

ash

≤0.60%

EEO

≤0.14

transition temp

gel point 41 °C ±1.5 °C (1.5% gel)

gel strength

≥900 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.20%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Agarose High Gelling Temperature

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A6877

Agarose

form

powder

form

powder

form

powder

form

powder

impurities

≤10% water

impurities

-

impurities

≤7% water

impurities

≤10% moisture content

EEO

≤0.14

EEO

≤0.05

EEO

≤0.12

EEO

0.16-0.19

transition temp

gel point 41 °C ±1.5 °C (1.5% gel)

transition temp

gel point 8-17 °C

transition temp

gel point ≤20 °C (1.5% gel)

transition temp

congealing temperature 34.5-37.5 °C (1.5% gel)

gel strength

≥900 g/cm2 (1% gel)

gel strength

-

gel strength

≥300 g/cm2 (1.5% gel)

gel strength

≥1000 g/cm2 (1.0% gel)

anion traces

sulfate (SO42-): ≤0.20%

anion traces

-

anion traces

sulfate (SO42-): ≤0.14%

anion traces

-

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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