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A7284

Sigma-Aldrich

Bovine Serum Albumin solution

30%±2% in 0.85% sodium chloride, aseptically filled

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Synonym(s):
Albumin solution from bovine serum, BSA solution
CAS Number:
MDL number:

biological source

bovine

Quality Level

sterility

aseptically filled

assay

28-32% protein basis (biuret)

form

solution

purified by

heat shock fractionation

contains

0.1% sodium azide as preservative

does not contain

stabilizer

origin

USA origin

concentration

28-32% protein (biuret)
30%±2% in 0.85% sodium chloride

technique(s)

ELISA: suitable

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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This Item
A1662A8327A3174
Bovine Serum Albumin solution 30%±2% in 0.85% sodium chloride, aseptically filled

A7284

Bovine Serum Albumin solution

Bovine Serum Albumin solution 30%±2% in 0.85% sodium chloride, aseptically filled

A1662

Bovine Serum Albumin solution

Bovine Serum Albumin solution 30%±2% in 0.85% sodium chloride, aseptically filled

A8327

Bovine Serum Albumin solution

Bovine Serum Albumin solution 30% in saline, high avidity, contains azide and caprylate, aseptically filled

A3174

Bovine Serum Albumin solution

technique(s)

ELISA: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable

technique(s)

blocking: suitable

assay

28-32% protein basis (biuret)

assay

28-32% protein basis (biuret)

assay

28-32% protein basis (biuret)

assay

28-32% (biuret)

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

200

form

solution

form

solution

form

solution

form

solution

General description

Bovine serum albumin (BSA) is bound in a single chain cross-linked with 17 cysteine residues. It comprises three homologous, structurally different domains and two sub-domains each. This globular, α-helical, non-glycosylated protein is produced in the liver.

Application

Bovine Serum Albumin solution has been used:
  • to study the effects of plasma protein on sulfated glycosaminoglycans (sGAGs)
  • to histologically evaluate the implanted allogeneic mesenchymal stem cells (MSCs) alginate constructs
  • as a component of phosphate-buffered saline (PBS) to treat red blood cells (RBC) for sample preparation

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the most widely used blocking agent in enzyme-linked immunosorbent assay (ELISA). It is also the main constituent of cell culture media. BSA promotes the differentiation of human embryonic stem cells (hESC) differentiation and assists the circulation of drugs, hormones, and fatty acids.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Mesenchymal stem cells (MSC) are promising candidates for use as a biological therapeutic. Since locally injected MSC disappear within a few weeks, we hypothesize that efficacy of MSC can be enhanced by prolonging their presence. Previously, encapsulation in alginate was
M J Leijs et al.
European cells & materials, 33, 43-58 (2017-02-01)
Bone marrow derived mesenchymal stem cells (MSCs) have immunomodulatory and trophic capacities. For therapeutic application in local chronic inflammatory diseases, MSCs, preferably of allogeneic origin, have to retain immunomodulatory properties. This might be achieved by encapsulation of MSCs in a

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