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A7431

Sigma-Aldrich

Agarose

BioReagent, for molecular biology, Wide range/Standard 3:1

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Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan
CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

biological source

algae (marine)

Quality Level

grade

for molecular biology

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

EEO

≤0.13

mp

≤90 °C

transition temp

gel point 32.5-38 °C (4% gel)

gel strength

≥1200 g/cm2 (4% gel)

anion traces

sulfate (SO42-): ≤0.15%

foreign activity

DNase, RNase, NICKase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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This Item
A9539A2929A5304
Agarose BioReagent, for molecular biology, Wide range/Standard 3:1

Sigma-Aldrich

A7431

Agarose

Essential+ Grade
Agarose BioReagent, for molecular biology, low EEO

Sigma-Aldrich

A9539

Agarose

Essential+ Grade
Agarose For pulsed field electrophoresis running gel

Sigma-Aldrich

A2929

Agarose

-
Agarose Special High EEO

Sigma-Aldrich

A5304

Agarose

-
grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

product line

BioReagent

product line

BioReagent

product line

-

product line

-

form

powder

form

powder

form

powder

form

powder

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable, immunoelectrophoresis: suitable

EEO

≤0.13

EEO

0.09-0.13

EEO

≤0.10

EEO

≤0.40

Application

Specially formulated to form strong, flexible gels for separation of small (≤1 kb) PCR products, DNA and RNA. Stronger gels make gel-handling easier. Sharp resolution is produced with DNA fragments ranging from 10-1500 bp.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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