MilliporeSigma
All Photos(1)

Documents

A7517

Sigma-Aldrich

Bovine Serum Albumin

For use as a marker in SDS-PAGE

Sign Into View Organizational & Contract Pricing

Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

bovine

Quality Level

Assay

24-32 mg/vial (biuret)

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol fractionation

packaging

vial of 25 mg

origin

USA origin

technique(s)

electrophoresis: suitable

pH

5.2

solubility

water: slightly soluble

suitability

suitable for (Suitable for use as an electrophoresis marker)

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A2934A9085A8022
Bovine Serum Albumin For use as a marker in SDS-PAGE

Sigma-Aldrich

A7517

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially globulin free, low endotoxin, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

A2934

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially IgG free, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A9085

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, pH 5.2, ≥96%

Sigma-Aldrich

A8022

Bovine Serum Albumin

assay

24-32 mg/vial (biuret)

assay

≥98% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

assay

≥96%

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

cold ethanol fractionation

purified by

heat shock fractionation

purified by

heat shock fractionation

purified by

heat shock fractionation

packaging

vial of 25 mg

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

General description

Bovine Serum Albumin (BSA) is a globular and α-helical single-chain protein, produced in the liver. This non-glycosylated protein belongs to the serum albumins family. BSA consists of three homologous domains with two sub-domains each.

Application

Bovine Serum Albumin (BSA) has been used:
  • as a standard protein in the elution-filter aided proteome preparation (FASP) method and in sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)
  • as a component of the molecular weight marker in SDS-PAGE and western blotting
  • as a blocking agent in immunofluorescence

Biochem/physiol Actions

Bovine Serum Albumin (BSA) participates in the transport of endogenous and exogenous substances, such as hormones, fatty acids, drugs, and xenobiotics. It is used as a blocking agent in enzyme-linked immunosorbent assay (ELISA). BSA is usually preferred for the preparation of serum-free media. It is used in the pharmaceutical and cosmetic industry.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 8

1 of 8

Bovine Serum Albumin lyophilized powder, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

A3733

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially fatty acid free and essentially globulin free, ≥99% (agarose gel electrophoresis)

Sigma-Aldrich

A0281

Bovine Serum Albumin

USP

USP

1076192

BSA for protein quantitation

Bovine Serum Albumin lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

A9418

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture

Sigma-Aldrich

A2058

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%

Sigma-Aldrich

A7030

Bovine Serum Albumin

Structural changes during the unfolding of Bovine serum albumin in the presence of urea: A small-angle neutron scattering study
Pramana, 63(2), 363-368 (2004)
Feiran Hao et al.
The Analyst, 141(16), 4953-4960 (2016-06-17)
Currently, the separation targets of preparative electrophoresis range from milligrams to micrograms of proteins. However, most commercially available preparative electrophoretic instruments function at the milligram level. Although some preparative electrophoretic apparatuses operated at the microgram level, the fractionation results are
Mehdi Fazlalipour et al.
Jundishapur journal of microbiology, 8(4), e17157-e17157 (2015-06-03)
Gradual development of a useful vaccine can be the main point in the control and eradication of Hepatitis C virus (HCV) infection. Hepatitis C Virus envelope glycoproteins are considered as the main HCV vaccine candidate. In this study, the Pichia
Subhajit Ghosh et al.
The journal of physical chemistry. B, 119(25), 7804-7815 (2015-05-30)
The study of protein-surfactant interactions is important because of the widespread use of surfactants in industry, medicine, and pharmaceutical fields. Sodium N-lauroylsarcosinate (SL-Sar) is a widely used surfactant in cosmetics, shampoos. In this paper, we studied the interactions of bovine
Yuhong Xiao et al.
Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service