A9056

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

Quality Level

biological source

bovine

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

application(s)

cell culture | mammalian: suitable

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Albumin is the most abundant plasma protein in humans. High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Serum albumin is a reliable prognostic indicator for liver disease. Oxidative damage of albumin is associated with advanced liver disease.

Application

Bovine Serum Albumin has been used as a supplement in Dulbecco′s modified eagle medium (DMEM). It has also been used in immunostaining of cell arrays.

Packaging

10, 50, 100, 500 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Suitability

Suitable for growth of Borrelia burgdorferi in BSK II medium

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Holger Erfle et al.
Nature protocols, 2(2), 392-399 (2007-04-05)
Here, we describe a robust protocol for the reverse transfection of cells on small interfering (siRNA) arrays, which, in combination with multi-channel immunofluorescence or time-lapse microscopy, is suitable for genome-wide RNA interference (RNAi) screens in intact human cells. The automatic...
Anna Szóstek-Mioduchowska et al.
Scientific reports, 10(1), 1119-1119 (2020-01-26)
Equine endometrial fibrosis (endometrosis) is described as a degenerative chronic condition in the uterus. Its characteristic feature is excessive deposition of extracellular matrix (ECM) components around the endometrial glands and stroma. Although matrix metallopeptidases (MMPs) that mediate ECM turnover are...
Oxidative damage of albumin in advanced liver disease
Oettl K, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1782(7-8), 469-473 (2008)
K K Piotrowska-Tomala et al.
Domestic animal endocrinology, 52, 90-99 (2015-05-04)
The aim of the study was to determine the effects of lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF), interleukin-1-alpha (IL-1α), and nitric oxide donor (NONOate) on both in vivo and in vitro secretion of prostaglandin (PG)E2, PGF2α, leukotriene (LT)B4, and...
The role of fresh cow's milk in iron deficiency: I. Albumin turnover in infants with iron deficiency anemia
Woodruff C W and Clark J L
American Journal of Diseases of Children, 124(1), 18-23 (1972)

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