A9918

Sigma-Aldrich

Agarose

Type II-A, Medium EEO

CAS Number:
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

biological source

algae (Gelidium)

Quality Level

type

Type II-A

form

powder

impurities

≤7% water

ash

≤0.5%

turbidity

≤40 NP (1.5% gel)

EEO

0.16-0.19

mp

86.5-89.5  °C

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

gel strength

≥1000 g/cm2 (1% gel)

anion traces

sulfate (SO42-): ≤0.25%

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Packaging

1 kg in poly bottle
25, 50, 100, 250, 500 g in poly bottle

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
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