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B2926

Millipore

Brucella Agar with Hemin and Vitamin K1

suitable for microbiology, NutriSelect® Plus

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NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

pH

7.0±0.2 (25 °C)

application(s)

clinical testing
food and beverages
veterinary

microbiology

suitability

anaerobic bacteria
nonselective for Bacteroides spp.
nonselective for Brucella spp.
nonselective for Clostridium spp.

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form

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form

powder

form

powder

form

powder

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbe id | specific enzyme detection: suitable, microbiological culture: suitable

pH

7.0±0.2 (25 °C)

pH

7.0±0.2 (25 °C)

pH

-

pH

7.4±0.2 (25 °C)

Application

Used for the isolation and subculture of anaerobes.

Components

Components (g/L):
Casein Enzymic Hydrolysate 10.00
Peptic Digest of Animal Tissue 10.00
Yeast Extract 2.00
Dextrose 1.00
Sodium Chloride 5.00
Sodium Bisulphite 0.10
Hemin 0.10
Vitamin K1 0.01
Agar 15.00

Preparation Note

Suspend 43.1 g of Brucella Agar with Hemin and Vitamin K1 in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add sterile 5% v/v defibrinated sheep blood.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sutter, V.L, et al.
Wadsworth Anaerobic Bacteriology Manual (1985)
W M Weinstein et al.
Infection and immunity, 10(6), 1250-1255 (1974-12-01)
An animal model has been developed to study the evolution of intra-abdominal abscesses. Gelatin capsules containing pooled colonic contents and barium sulfate were prepared in an anaerobic chamber and implanted into the pelvic region of Wistar rats. The natural course
A B Onderdonk et al.
Infection and immunity, 10(6), 1256-1259 (1974-12-01)
An animal model simulating intra-abdominal sepsis was produced by implanting large bowel contents into the pelvic region of rats. Bacteriological analysis of infected sites showed quantitative differences according to the stage of disease. During the initial, often lethal, peritonitis stage

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