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B4501

Sigma-Aldrich

Biotin

≥99% (HPLC), lyophilized powder

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Synonym(s):
D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
Empirical Formula (Hill Notation):
C10H16N2O3S
CAS Number:
Molecular Weight:
244.31
Beilstein:
86838
EC Number:
MDL number:
eCl@ss:
34058011
PubChem Substance ID:
NACRES:
NA.21

biological source

synthetic (organic)

Quality Level

Assay

≥99% (HPLC)

form

lyophilized powder

technique(s)

HPLC: suitable

color

white to off-white

mp

231-233 °C (lit.)

solubility

ammonium hydroxide: 50 mg/mL (2 M)

storage temp.

2-8°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2

InChI

1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1

InChI key

YBJHBAHKTGYVGT-ZKWXMUAHSA-N

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This Item
B030114400B4639
Biotin ≥99% (HPLC), lyophilized powder

Sigma-Aldrich

B4501

Biotin

-
Biotin meets USP testing specifications

Sigma-Aldrich

B0301

Biotin

-
Biotin ≥99.0% (T)

Sigma-Aldrich

14400

Biotin

-
Biotin powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

Sigma-Aldrich

B4639

Biotin

Essential+ Grade
assay

≥99% (HPLC)

assay

97.5-102.0%

assay

≥99.0% (T)

assay

≥99%

form

lyophilized powder

form

powder

form

powder

form

powder

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

color

white to off-white

color

white

color

colorless to white

color

white to off-white

mp

231-233 °C (lit.)

mp

231-233 °C (lit.)

mp

229-234 °C, 231-233 °C (lit.)

mp

231-233 °C (lit.)

General description

Biotin is a water-soluble vitamin. Mammals depend on biotin via plant and microbial dietary sources. Biotin consists of two rings that are attached to valeric acid moiety as a side chain. One of the rings is attached to ureido group and the other ring is the tetrahydrothiophene group.

Application

Biotin may be used to elute proteins from avidin/streptavidin resins. Biotin has been used:
  • for culturing of oligodendrocytes.
  • as a vitamin supplement for the growth of Bacillus species.
  • for blocking endogenous biotin during immunohistology procedures.

Biochem/physiol Actions

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are is required for various applications. Biotin also works as a cofactor/coenzyme of carboxylating enzymes, such as pyruvate carboxylase, acetyl-CoA carboxylases 1 and 2, 3-methylcrotonyl-CoA carboxylase (MCC), and propionyl-CoA carboxylase (PCC). It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A, etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry, and flow cytometry. This approach is also applied for to the purification and characterization of various molecules of interest. Biotin is involved in the epigenetic regulation of genes, cell signaling, and the structure of chromatin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The biotin-(strept)avidin system: principles and applications in biotechnology.
Diamandis EP and Christopoulos TK
Clinical Chemistry, 37, 625-625 (1991)
Lone Frydelund-Larsen et al.
Experimental physiology, 92(1), 233-240 (2006-10-13)
Exercise induces a marked increase in interleukin-8 (IL-8) mRNA and protein expression within skeletal muscle fibres. Interleukin-8 belongs to a subfamily of CXC chemokines containing a Glu-Leu-Arg (ELR) motif. CXC chemokines with ELR motifs are potent angiogenic factors in vivo
Biotin: biochemical, physiological and clinical aspects
Said H M
Sub-Cellular Biochemistry, 56, 1-19 (2012)
Isolation and culture of rat and mouse oligodendrocyte precursor cells.
Chen Y et al.
Nature Protocols, 2, 1044-1044 (2007)
Yuan He et al.
Nature, 530(7590), 354-357 (2016-01-28)
Inflammasomes are intracellular protein complexes that drive the activation of inflammatory caspases. So far, four inflammasomes involving NLRP1, NLRP3, NLRC4 and AIM2 have been described that recruit the common adaptor protein ASC to activate caspase-1, leading to the secretion of

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