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Benzamidine hydrochloride hydrate


Benzenecarboximidamide hydrochloride, Benzamidinium chloride, Amidinobenzene hydrochloride
Linear Formula:
C6H5C(=NH)NH2 · HCl · xH2O
CAS Number:
Molecular Weight:
156.61 (anhydrous basis)
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

biological source

synthetic (organic)






86-88 °C (lit.)


water: 50 mg/mL, clear to slightly hazy, colorless to faintly yellow

storage temp.


SMILES string




InChI key


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Benzamidine hydrochloride hydrate has been used:
  • in western blot analysis
  • in immunoprecipitation
  • in the purification of yeast dynein
  • as a protease inhibitor in the preparation of brush-border membrane vesicles
  • for the processing of human synovial fluid, blood and eye sleep
  • to prevent non-specific degradation of cartilages


5, 25, 100 g in glass bottle

Biochem/physiol Actions

Peptidase inhibitor.
Benzamidine HCl is a reversible inhibitor of trypsin, trypsin-like enzymes, and serine proteases. A concentration of approximately 1 mM is used for general protease inhibition. To inhibit proteases from yeast, a range of 0.5 to 4.0 mM is used and it is for the most part interchangeable with pepstatin A.

In addition to being a strong competitive inhibitor of trypsin, benzamidine HCl has been also shown to be a strong competitive inhibitor of thrombin and plasmin. It was also found to be as effective as aprotinin in the prevention of glucagon degradation in human plasma.

Preparation Note

Benzamidine HCl is soluble in water and alcohol. Solubility testing in water at a concentration of 50 mg/ml yields a clear solution with heating. Benzamidine HCl is sensitive to oxidation. It is recommended to prepare solutions fresh each time in degassed water prior to use. However, frozen aliquots stored under inert gas, to exclude air, may be stable for a short time. Insufficient information is available to assess the shelf-life of a frozen solution.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Contrast-enhanced CT imaging as a non-destructive tool for ex vivo examination of the biochemical content and structure of the human meniscus
Oh DJ, et al.
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, 35(5), 1018-1028 null
Sialidase unmasks mucin domain epitopes of lubricin
Solka KA, et al.
The Journal of Histochemistry and Cytochemistry, 64(11), 647-668 (2016)
Contrast-enhanced CT using a cationic contrast agent enables non-destructive assessment of the biochemical and biomechanical properties of mouse tibial plateau cartilage
Lakin BA, et al.
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, 34(7), 1130-1138 (2016)
The MDM2 RING and central acidic domains play distinct roles in MDM2 homodimerization and MDM2-MDMX heterodimerization
Leslie PL, et al
The Journal of biological chemistry, 6(51), jbc-M115 (2015)
Combining Structure-Function and Single-Molecule Studies on Cytoplasmic Dynein
Single Molecule Analysis, 53-89 (2018)

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