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MilliporeSigma

B8522

Brilliant Blue G solution

Concentrate

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About This Item

CAS Number:
6104-58-1
MDL number:
MFCD00078482
UNSPSC Code:
12171500
PubChem Substance ID:
24892075
NACRES:
NA.47

Key Documents

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form

liquid

technique(s)

microbe id | staining: suitable

color

dark blue

suitability

suitable for (Suitable for for staining protein in polyacrylamide and agarose gels)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

InChI key

RWVGQQGBQSJDQV-UHFFFAOYSA-M

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Show Differences

1 of 4

This Item
B2025B6529B0770
Brilliant Blue G solution Concentrate

Sigma-Aldrich

B8522

Brilliant Blue G solution

Brilliant Blue G - Colloidal Concentrate

Sigma-Aldrich

B2025

Brilliant Blue G - Colloidal Concentrate

Brilliant Blue R Staining Solution suitable for (for immunoelectrophoresis protein staining)

Sigma-Aldrich

B6529

Brilliant Blue R Staining Solution

Brilliant Blue G pure

Sigma-Aldrich

B0770

Brilliant Blue G

suitability

suitable for (Suitable for for staining protein in polyacrylamide and agarose gels)

suitability

-

suitability

suitable for (for immunoelectrophoresis protein staining)

suitability

-

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

color

dark blue

color

-

color

dark blue

color

dark violet

form

liquid

form

solution

form

liquid

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Brilliant Blue G is a methanol based stain which is especially designed for staining protein in polyacrylamide gels, but will also stain protein in agarose gels (IEF). The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Water is added directly to the bottle to produce 1 liter of ready-to-use staining solution.[1]

Application

Brilliant Blue G solution has been used for protein band detection after SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It is also used for the protein staining of cells on microscopic slide.[2][3]

Preparation Note

After dilution with deionized water to a final volume of one liter, solution will contain 0.1% (w/v) Brilliant Blue G, 25% (v/v) methanol and 5% (v/v) acetic acid.
One bottle equals one liter working solution. 300 ml of brilliant blue G solution concentrate is supplied in the bottle.

Analysis Note

This product has been tested for suitability on SDS-PAGE.

related product

Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

49.5 °F - closed cup

flash_point_c

9.7 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000419649
0000419649
SLCN1989
SLCK9977
SLCF2517

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Suma Tiruvayipati et al.
PloS one, 15(3), e0228871-e0228871 (2020-03-03)
Exosomes are extracellular vesicles (EVs) of ~20-200 nm diameter that shuttle DNAs, RNAs, proteins and other biomolecules between cells. The large number of biomolecules present in exosomes demands the frequent use of high-throughput analysis. This, in turn, requires technical replicates
Effect of an experimental proteasome inhibitor on the cytoskeleton, cytosolic protein turnover, and induction in the neuronal cells in vitro.
Csizmadia V, et al.
Neurotoxicology, 29, 232-232 (2008)
Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane.
Biochimica et Biophysica Acta, 1864, 303-303 (2017)
Ahmed H
Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
Hyung-Keun Ku et al.
Analytical biochemistry, 434(1), 178-180 (2012-12-04)
The Bradford assay is a simple method for protein quantitation, but variation in the results between proteins is a matter of concern. In this study, we compared and normalized quantitative values from two models for protein quantitation, where the residues
View All Related Papers

Articles

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Questions

  1. Does this product B8522 require a destaining step?

    1 answer

    1. Yes. It is necessary to destain after staining with product B8522.
      For staining proteins in polyacrylamide gels and agarose gels Brilliant Blue G can be prepared in a deionized water solution containing the following: 0.1% (w/v) Brilliant Blue G, 25% (v/v) methanol, and 5% (v/v) acetic acid. This staining solution can easily be prepared from Product No. B8522, which is a solution of Brilliant Blue G in methanol/acetic acid. Add water to a final volume of 1 liter.

      Procedure for staining IEF gels.
      1. Rinse the gel several times with deionized water.
      2. Incubate in staining solution for about one hour at room temperature.
      3. Prepare the destaining solution by combining 750 ml of methanol (Product No. M3641), 150 ml of glacial acetic acid (Product No. A6283), and 2.1 liters of deionized water.
      4. Incubate the gel with destaining solution until the gel is clear, changing the solution frequently. The
      times for staining and destaining will vary with the size of the gel and other factors.

      Helpful?

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