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MilliporeSigma

B8756

Bile salts

suitable for microbiology

Synonym(s):

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture

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10 G

$35.20

50 G

$113.00

100 G

$194.00

500 G

$802.00

$35.20


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About This Item

UNSPSC Code:
41106212
NACRES:
NA.85

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sterility

non-sterile

Quality Level

form

powder

solubility

distilled water: freely soluble

application(s)

microbiology

storage temp.

10-30°C

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This Item
48305B3883D6750
application(s)

microbiology

application(s)

microbiology

application(s)

microbiology

application(s)

-

form

powder

form

powder

form

dried, unfractionated

form

powder

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

-

storage temp.

10-30°C

storage temp.

10-30°C

storage temp.

-

storage temp.

room temp

solubility

distilled water: freely soluble

solubility

H2O: 0.1 g/mL, clear, faintly yellow

solubility

water: soluble 50 mg/mL

solubility

water: 50 mg/mL, clear, colorless to light yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Bile salt is an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Biles salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic and lithocholic acids.Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that enables isolation and identification of pathogens. For example, in MacConkey Agar/Broth.

Application

Bile salts may be used as one of the selective agents to prevent the growth of injured Escherichia coli cells, in an experiment done in order to identify the specific agent in the selective media which would inhibit the growth of the injured Escherichia coli ML30 cells.[1] It may also be used for identification of isolates of Vibrio (Beneckea) vulnificus which is a halophilic bacterium, found responsible for causing several human infections.[2]

Other Notes

Mixture of sodium cholate and sodium deoxycholate

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Lian-Hua Cui et al.
Journal of microbiology and biotechnology, 28(4), 510-519 (2018-02-01)
Synbiotics are a combination of probiotics and prebiotics, which lead to synergistic benefits in host welfare. Probiotics have been used as an alternative to antibiotics. Among the probiotics, Pediococcus acidilactici (PA) has shown excellent antimicrobial activity against Salmonella Gallinarum (SG)
Divya Eratte et al.
Food chemistry, 227, 129-136 (2017-03-10)
Solid co-microcapsules of omega-3 rich tuna oil and probiotic bacteria L. casei were produced using whey protein isolate-gum Arabic complex coacervate as wall material. The in-vitro digestibility of the co-microcapsules and microcapsules was studied in terms of survival of L.
Christina S Faherty et al.
Molecular microbiology, 85(1), 107-121 (2012-05-11)
Shigella flexneri is a Gram-negative pathogen that invades the colonic epithelium. While invasion has been thoroughly investigated, it is unknown how Shigella first attaches to the epithelium. Previous literature suggests that Shigella utilizes adhesins that are induced by environmental signals
Daniela Andrea Ramirez et al.
Food chemistry, 215, 493-500 (2016-08-21)
Organosulphur compounds (OSCs) present in garlic (Allium sativum L.) are responsible of several biological properties. Functional foods researches indicate the importance of quantifying these compounds in food matrices and biological fluids. For this purpose, this paper introduces a novel methodology
D L Scheusner et al.
Applied microbiology, 21(1), 46-49 (1971-01-01)
A population of Escherichia coli ML30 cells was exposed to a quaternary ammonium compound, and injury to the cells was measured by a comparison of counts on Trypticase Soy Agar and Violet Red Bile Agar. Substantial injury could not be

Questions

1–2 of 2 Questions  
  1. What is the molecular weight of B8756?

    1 answer
    1. B8756 is composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic and lithocholic acids. Since this product is a mix of bile salts, there is not a molecular weight for the product. You can use the molecular weight of each component and average it - but that will not allow for accuracy in making molar solutions.

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  2. Is it used to mimic the physical and physiological properties of bile ?

    1 answer
    1. Yes, bile salts can be used to mimic the physical and physiological conditions of bile in certain applications, but does not reflect the full panel of Bile components. Bile is typically composed of 0.7% bile salts, 0.2% bilirubin, 0.51% fats (cholesterol, fatty acids, and lecithin), and 200 meq/L inorganic salts.

      Helpful?

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