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C0537

Sigma-Aldrich

5-Carboxyfluorescein

99% (HPLC)

Synonym(s):
5-FAM
Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
Molecular Weight:
376.32
Beilstein:
57037
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

99% (HPLC)

form

powder

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

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Application

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

Packaging

50 mg in poly bottle

Biochem/physiol Actions

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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