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C1144

Sigma-Aldrich

Chlorazol Black

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Synonym(s):
4-Amino-3-[[4′-[(2,4-diaminophenyl)azo][1,1′-biphenyl]-4-yl]azo]-5-hydroxy-6-(phenylazo)-2,7-naphthalenedisulfonic acid disodium salt, Chlorazole Black E, Direct Black 38
Empirical Formula (Hill Notation):
C34H25N9Na2O7S2
CAS Number:
Molecular Weight:
781.73
Colour Index Number:
30235
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, ≥45% (based on Nitrogen)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Nc1ccc(\N=N\c2ccc(cc2)-c3ccc(cc3)\N=N\c4c(N)c5c(O)c(\N=N\c6ccccc6)c(cc5cc4S([O-])(=O)=O)S([O-])(=O)=O)c(N)c1

InChI

1S/C34H27N9O7S2.2Na/c35-22-10-15-27(26(36)18-22)41-38-24-11-6-19(7-12-24)20-8-13-25(14-9-20)40-42-32-28(51(45,46)47)16-21-17-29(52(48,49)50)33(34(44)30(21)31(32)37)43-39-23-4-2-1-3-5-23;;/h1-18,44H,35-37H2,(H,45,46,47)(H,48,49,50);;/q;2*+1/p-2/b41-38+,42-40+,43-39+;;

InChI key

XRPLBRIHZGVJIC-UVHCWRHYSA-L

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1 of 4

This Item
N9002F7253O0625
Chlorazol Black

Sigma-Aldrich

C1144

Chlorazol Black

Naphthol Blue Black Dye content ~50 %

Sigma-Aldrich

N9002

Naphthol Blue Black

Oil Red O certified by the Biological Stain Commission

Sigma-Aldrich

O0625

Oil Red O

composition

Dye content, ≥45% (based on Nitrogen)

composition

Dye content, ~50%

composition

Dye content, ≥25%

composition

Dye content, ≥75%

solubility

H2O: 1 mg/mL

solubility

water: 1 mg/mL

solubility

water: 10 mg/mL

solubility

chloroform/ethanol (1:1): 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Chlorazol Black has strong affinity for cellulose and chitin. In plant tissues, it helps to differentiate between cellulose and lignin. It also helps in distinguishing fungal bodies from artefacts due to chitin staining. It is also used as an auxiliary stain for chromosomes.

Application

Chlorazol Black has been used to monitor the infection of mycorrhizal Allium porrum in plant roots. It has also been used for the staining of copepods.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B - Eye Irrit. 2 - Repr. 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Asterocheres hirsutus, a new species of parasitic copepod (Siphonostomatoida: Asterocheridae) associated with an Antarctic hexactinellid sponge.
Bandera M, et al.
Helgoland Marine Research , 59, 315-315 (2005)
A Dewan et al.
Archives of environmental health, 43(4), 269-272 (1988-07-01)
Urine samples of workers in a small-scale unit manufacturing Direct Black 38 were analyzed by high-pressure liquid chromatography for the presence of benzidine and mono and diacetyl benzidine. Acetylated metabolites were found in all the urine samples, and benzidine was
Ticiane Pokrywiecki Sauer et al.
Journal of hazardous materials, 135(1-3), 274-279 (2006-01-03)
The application of advanced oxidation processes (H(2)O(2)/UV, TiO(2)/H(2)O(2)/UV and TiO(2)/UV) to treat tannery wastewater was investigated. The experiments were performed in batch and continuous UV reactors, using TiO(2) as a catalyst. The effect of the hydrogen peroxide concentration on the
Notes on Microscopical Technique for Zoologists null
F D Aldrich et al.
Journal of toxicology and environmental health, 18(3), 347-355 (1986-01-01)
A benzidine-derived azo dye, C.I. direct black 38 (DB38), and a p-phenylenediamine-derived dye, C.I. direct black 19 (DB19), labeled with carbon-14 in their aromatic amine moieties, were applied to the shaved dorsal skin of male Fischer-344 rats and New Zealand

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