C1144

Chlorazol Black

Powder

• Synonym(s): 4-Amino-3-[[4′-[(2,4-diaminophenyl)azo][1,1′-biphenyl]-4-yl]azo]-5-hydroxy-6-(phenylazo)-2,7-naphthalenedisulfonic acid disodium salt, Chlorazole Black E, Direct Black 38
• Empirical Formula (Hill Notation): C₃₄H₂₅N₉Na₂O₇S₂
• CAS Number: 1937-37-7
• Molecular Weight: 781.73
• NACRES: NA.47
• PubChem Substance ID: 24892361
• UNSPSC Code: 12171500
• EC Number: 217-710-3
• MDL number: MFCD00066363
• Colour Index Number: 30235

Properties

• Product Name: Chlorazol Black,
• InChI key: XRPLBRIHZGVJIC-UVHCWRHYSA-L
• InChI: 1S/C34H27N9O7S2.2Na/c35-22-10-15-27(26(36)18-22)41-38-24-11-6-19(7-12-24)20-8-13-25(14-9-20)40-42-32-28(51(45,46)47)16-21-17-29(52(48,49)50)33(34(44)30(21)31(32)37)43-39-23-4-2-1-3-5-23;;/h1-18,44H,35-37H2,(H,45,46,47)(H,48,49,50);;/q;2*+1/p-2/b41-38+,42-40+,43-39+;;
• SMILES string: [Na+].[Na+].Nc1ccc(\N=N\c2ccc(cc2)-c3ccc(cc3)\N=N\c4c(N)c5c(O)c(\N=N\c6ccccc6)c(cc5cc4S([O-])(=O)=O)S([O-])(=O)=O)c(N)c1
• form: powder
• composition: Dye content, ≥45% (based on Nitrogen)
• color: dark brown to black
• solubility: H₂O: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• Quality Level: 200

Description

General description

Chlorazol Black has strong affinity for cellulose and chitin. In plant tissues, it helps to differentiate between cellulose and lignin.[1] It also helps in distinguishing fungal bodies from artefacts due to chitin staining.[2] It is also used as an auxiliary stain for chromosomes.[3]

Application

Chlorazol Black has been used to monitor the infection of mycorrhizal Allium porrum in plant roots.[4] It has also been used for the staining of copepods.[5]

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H319,H350,H361d
• pcodes: P202 - P264 - P280 - P305 + P351 + P338 - P308 + P313 - P337 + P313
• Hazard Classifications: Carc. 1B - Eye Irrit. 2 - Repr. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type P2 (EN 143) respirator cartridges