The recommended long term storage temperature for the product is -20°C. No formal studies have been performed to determine how many times the solution can be thawed and refrozen at -20°C, nor is solution stability data available for the product once it has been diluted. Given this absence of data, it would be best to store the product in single use aliquots.
C1359
Calcein AM solution
≥90% purity (HPLC), solution
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About This Item
Empirical Formula (Hill Notation):
C46H46N2O23
CAS Number:
Molecular Weight:
994.86
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
200-664-3
MDL number:
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Product Name
Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution
InChI key
XKFSBWQWNMZWFA-UHFFFAOYSA-N
InChI
1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
SMILES string
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
assay
≥90% (HPLC)
form
solution
concentration
4 mM in DMSO
technique(s)
titration: suitable
color
colorless, white to off-white
λmax
491-497 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
Quality Level
1 of 4
This Item | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) - | technique(s) - | technique(s) - |
| concentration 4 mM in DMSO | concentration - | concentration - | concentration - |
| color colorless, white to off-white | color - | color - | color off-white |
| form solution | form - | form - | form solid |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) - |
| assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥95.0% (HPLC) | assay ≥90% (HPLC) |
Application
In cells, calcein AM solution has been used in calcein-AM dye transfer assay. It has been used for the staining of cells to study quantification of cell-migration. It has been used to study cytotoxic effects of drugs in cells.[1]
Biochem/physiol Actions
Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.
Fluorescent cell permeable derivative of calcein.
Physical form
Solution in 0.4% Calcein AM and 99.6% DMSO.
related product
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
185.0 °F - closed cup
flash_point_c
85 °C - closed cup
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Once thawed, can the solution be frozen again? If yes, should the aliquots be made?
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