This product is in powder form. The material is not tested for solubility, however, other collagenase enzymes currently offered are typically prepared at 1-2 mg/mL in TESCA buffer (containing 50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37 C. The minimum activity of this product is 125 units per milligram. The activity is lot specific and reported in the Certificate of Analysis. Please see the link below to access the required Certificate:
https://www.sigmaaldrich.com/search
C1-BIOC
Collagenase Type I, Cls I
Synonym(s):
Collagen A
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 mg | Check Cart for Availability | $59.90 | |
| 1 g | Check Cart for Availability | $370.00 |
About This Item
NACRES:
NA.78
UNSPSC Code:
12352204
General description
This product is identical to Biochrom GmbH part numbers C1-22 and C1-28. Enzyme blends of collagenase, clostripain, with tryptic and proteolytic activities from Clostridium histolyticum. This preparation is recommended for liver, lung, adipose, and adrenal tissue. Specific activity is 125 to 250 Mandl units per ml of powder substance.
Collagenase Type I (Cls I) is a tissue dissociation enzyme produced by Clostridium histolyticum. It digests large, intact triple helices of native collagen fibers. Collagenases belong to the matrix metalloproteinase (MMPs) family that digest collagens in the extracellular matrix (ECM). Collagens serve to be a major component of the extracellular matrix and in mammals. These structural proteins are triple helixes composed of three collagen polypeptides with high tensile strength.
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This Item | |||
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
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Tissue dissociation enzymes for adipose stromal vascular fraction cell isolation: a review
Lockhart RA, et al.
Journal of stem cell research & therapy (2015)
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Sehr geehrte Damen und Herren, in wie viel µl wird denn die Kollagenase Typ I (100mg) gelöst? Vielen Dank und liebe Grüße Verena
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