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C2734

Sigma-Aldrich

5-Carboxytetramethylrhodamine

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Synonym(s):
5-TAMRA
Empirical Formula (Hill Notation):
C25H22N2O5
CAS Number:
91809-66-4
Molecular Weight:
430.45
MDL number:
MFCD01073638
NACRES:
NA.47

Assay

≥95% (HPLC)

Quality Level

100

form

solid

ε (extinction coefficient)

≥85000-97000 at 539-545 nm in methanol

fluorescence

λex ~543 nm; λem ~570 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

InChI key

YMZMTOFQCVHHFB-UHFFFAOYSA-N

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This Item
T5428219538.51026
5-Carboxytetramethylrhodamine

Sigma-Aldrich

C2734

5-Carboxytetramethylrhodamine

Tetramethylrhodamine methyl ester perchlorate ≥95%

Sigma-Aldrich

T5428

Tetramethylrhodamine methyl ester perchlorate

5(6)-Carboxytetramethylrhodamine BioReagent, suitable for fluorescence, ≥85% (sum of isomers, HPCE)

Sigma-Aldrich

21953

5(6)-Carboxytetramethylrhodamine

5-Carboxy-tetramethylrhodamine Novabiochem®

Sigma-Aldrich

8.51026

5-Carboxy-tetramethylrhodamine

form

solid

form

powder

form

solid

form

powder

ε (extinction coefficient)

≥85000-97000 at 539-545 nm in methanol

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

fluorescence

λex ~543 nm; λem ~570 nm in methanol

fluorescence

λem 573 nm in methanol

fluorescence

λex 543 nm; λem 572 nm in methanol

fluorescence

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

peptide synthesis

storage temp.

room temp

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

General description

An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

Application

5-Carboxytetramethylrhodamine has been used in fluorophore characterization.

Biochem/physiol Actions

5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
Pack Size/Quantity

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705578-5MG-PW

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MMYOMAG-74K-13

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