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C3023

Sigma-Aldrich

CHAPS hydrate

≥98% (HPLC)

Synonym(s):
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
Molecular Weight:
614.88 (anhydrous basis)
PubChem Substance ID:
NACRES:
NA.21

Quality Level

description

zwitterionic

assay

≥98% (HPLC)

form

powder

mol wt

614.88 g/mol (anhydrous basis)

technique(s)

dialysis: suitable
electrophoresis: suitable

impurities

<0.04% DMF

CMC

8 mM

mp

157 °C (dec.) (lit.)

transition temp

cloud point >100 °C

SMILES string

O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1

InChI key

SJCUTFKCLFLIFE-JWTJKVBLSA-N

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General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.
A solution at 50 mg/ml in water yields a clear-colorless solution. Aqueous solutions of CHAPS can be autoclaved.

Application

0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.

Packaging

1, 5, 25, 100 g in poly bottle

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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More Documents

Quotes and Ordering

Brain-derived neurotrophic factor of the cerebral microvasculature: a forgotten and nitric oxide-dependent contributor of brain-derived neurotrophic factor in the brain.
Monnier A, et al.
Acta Physiol., 219(4), 790-802 (2017)
Identification of a scavenger receptor homologue on nonspecific cytotoxic cells and evidence for binding to oligodeoxyguanosine.
Kaur H, et al.
Fish & Shellfish Immunology, 15(3), 169-181 (2003)
A Monnier et al.
Acta physiologica (Oxford, England), 219(4), 790-802 (2016-07-02)
Evidence that brain-derived neurotrophic factor (BDNF), a neurotrophin largely involved in cognition, is expressed by cerebral endothelial cells led us to explore in rats the contribution of the cerebral microvasculature to BDNF found in brain tissue and the link between
Procedures for whole-mount immunohistochemistry and in situ hybridization of immature mammalian CNS.
Luque JM, et al.
Brain Res. Protoc., 2(2), 165-173 (1998)
Eric M Wexler et al.
Stem cells (Dayton, Ohio), 27(5), 1130-1141 (2009-05-07)
Wnt signaling regulates neural stem cell (NSC) function and development throughout an individual's lifetime. Intriguingly, adult hippocampal progenitors (AHPs) produce several Wnts, and the intracellular machinery necessary to respond to them, creating the potential for an active autocrine-signaling loop within

Protocols

Solubilization of Membrane Proteins

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