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C3023

Sigma-Aldrich

CHAPS hydrate

≥98% (HPLC)

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Synonym(s):
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
Molecular Weight:
614.88 (anhydrous basis)
PubChem Substance ID:
NACRES:
NA.21

biological source

bovine bile

Quality Level

description

zwitterionic

Assay

≥98% (HPLC)

form

powder

mol wt

614.88 g/mol (anhydrous basis)

technique(s)

dialysis: suitable
electrophoresis: suitable

impurities

<0.04% DMF

CMC

8 mM

mp

157 °C (dec.) (lit.)

transition temp

cloud point >100 °C

SMILES string

O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1

InChI key

SJCUTFKCLFLIFE-JWTJKVBLSA-N

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This Item
C5070C9426CHAPS-RO
CHAPS hydrate &#8805;98% (HPLC)

Sigma-Aldrich

C3023

CHAPS hydrate

-
CHAPS hydrate BioXtra, &#8805;98% (TLC)

Sigma-Aldrich

C5070

CHAPS hydrate

Premium Grade
CHAPS hydrate BioReagent, suitable for electrophoresis, &#8805;98% (HPLC)

Sigma-Aldrich

C9426

CHAPS hydrate

Essential+ Grade
CHAPS 98% (from N), suitable for dialysis, solubility: &gt;50% (Aqueous solutions)

Roche

CHAPS-RO

CHAPS

-
description

zwitterionic

description

zwitterionic

description

zwitterionic

description

zwitterionic

assay

≥98% (HPLC)

assay

≥98% (TLC)

assay

≥98% (HPLC)

assay

98% (from N)

form

powder

form

powder

form

powder

form

crystals

mol wt

614.88 g/mol (anhydrous basis)

mol wt

614.88 g/mol (anhydrous basis)

mol wt

614.88 g/mol (anhydrous basis)

mol wt

614.88 g/mol (anhydrous basis)

technique(s)

dialysis: suitable, electrophoresis: suitable

technique(s)

protein purification: suitable, protein quantification: suitable

technique(s)

electrophoresis: suitable, protein quantification: suitable

technique(s)

dialysis: suitable

General description

CHAPS hydrate, a zwitterionic detergent derived from Cholic acid, stands out as an excellent tool in biochemical and cell biology research, particularly in the solubilization of membrane proteins. This versatile detergent, combining features of sulfobetaine-type and bile-acid detergents, exhibits a remarkable solubility rate of over 50% in aqueous solutions. It serves a pivotal role in stabilizing protein-DNA complexes, ensuring the retention of biochemical activity in solution.

Widely utilized as a buffer in various research applications, CHAPS hydrate acts as an amphiphilic surfactant, proving effective in solubilizing plasma membrane proteins and diluting assay components. Notably, its easy removal through dialysis or gel filtration adds to its appeal, making it a preferred choice for molecular mass determination by fluorometry. This feature underscores CHAPS hydrate′s adaptability in diverse experimental setups. However, caution is advised in assays involving polyvalent metals or metal ions sensitive to nonspecific chelating agents. Beyond its conventional use, CHAPS hydrate finds application as a polymer matrix in binding assays, effectively removing metal ions from protein solutions.

Application

0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.

Features and Benefits

  • Suitable for Biochemical and Cell Biology research
  • Can be used in Dialysis and Electrophoresis
  • High purity product for research applications

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Protocols

Solubilization of Membrane Proteins

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