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MilliporeSigma

C9386

Millipore

Casein Acid Hydrolysate for Biotechnology and Microbiology

for Biotechnology and Microbiology, ≥12.5% Total N, 8-12% Amino

Synonym(s):

Casein hydrolyzates, Hy-Case® SF, Peptone from casein, casein acid hydrolysate

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106212
NACRES:
NA.85

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Product Name

Hy-Case® SF, Casein acid hydrolysate, from bovine milk

biological source

bovine milk

Quality Level

form

powder

nitrogen analysis

8-12% amino, ≥12.5% total

loss

≤11% loss on drying

pH

5.4-7.4

application(s)

food and beverages
microbiology

storage temp.

10-30°C

suitability

Streptococcus spp.
Streptomyces spp.

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This Item
C7970A242770169
Amicase® Casein acid hydrolysate, from bovine milk

A2427

Amicase®

biological source

bovine milk

biological source

bovine milk

biological source

bovine milk

biological source

bovine milk

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

application(s)

food and beverages
microbiology

application(s)

food and beverages
veterinary, microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages, microbiology

nitrogen analysis

8-12% amino, ≥12.5% total

nitrogen analysis

≥4.6% amino, ≥7% total

nitrogen analysis

≥9% amino, ≥12% total

nitrogen analysis

-

storage temp.

10-30°C

storage temp.

-

storage temp.

15-25°C

storage temp.

-

suitability

Streptococcus spp., Streptomyces spp.

suitability

-

suitability

Bacillus spp., Enterobacter spp., Escherichia coli, Saccharomyces spp., Staphylococcus spp., Streptomyces spp., coliforms

suitability

-

General description

Hy-Case® SF is a high-quality acid hydrolysis of casein. Casein is a rich source of amino nitrogen so on treatment with a strong acid, it gets hydrolysed, producing free amino acids and several short peptide fragments. Special processing keeps inorganic ions to a minimum, so Hy-Case® SF is virtually a high concentration of free amino acids with a minimum of salt. It is a refined grade hydrolysate with high clarity in solution and is food grade and is recommended where a mixture of highly soluble free amino acids is required. It is especially recommended when inorganic ion levels need to be kept at a minimum.

Application

Casein hydrolysate Hy-Case® SF was used in DMCY medium, during an experimental study conducted to investigate the role of ssgA gene product in promoting the sporulation and cell division in Streptomyces griseus.[1] It may also be used in the YH medium in studies related to Halobacterium denitrificans sp. which is characterised as being carbohydrate-utilizing, denitrifying, extremely halophilic bacteria.[2]
Recommended for use as a microbiological nutrient in laboratory media, fermentations, and tissue culture applications requiring a mixture of highly soluble free amino acids.

Linkage

Similar to Hy-Case Amino, but contains a maximum of 2.0% sodium chloride

Reconstitution

Recommended concentration: 25 g/L

Legal Information

Hy-Case is a registered trademark of Kerry Group

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Questions

1–2 of 2 Questions  
  1. How are the Amicase® (product A2427) and Hy-Case® SF (product C9386) different?

    1 answer
    1. The Amicase® (product A2427) and Hy-Case® SF (product C9386) are related items, but they are not exactly the same.  The Amicase® is prepared to keep the inorganic ions to a minimum so it is high in total nitrogen.  This is also the case for the Hy-Case® SF; however, this item is prepared to be essentially salt free (contains a maximum of 2.0% sodium chloride).  The Hy-Case® SF is recommended for use as a microbiological nutrient in laboratory media and fermentations, where a mixture of highly soluble free amino acids is required. It is especially recommended when inorganic ion levels need to be kept at a minimum.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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