D8001

3,3′-Diaminobenzidine

≥97% (HPLC)

• Synonym(s): 3,3′,4,4′-Biphenyltetramine, 3,3′,4,4′-Tetraaminobiphenyl, DAB
• Linear Formula: (NH₂)₂C₆H₃C₆H₃(NH₂)₂
• CAS Number: 91-95-2
• Molecular Weight: 214.27
• Beilstein: 1212988
• EC Number: 202-110-6
• MDL number: MFCD00007725
• PubChem Substance ID: 24894175
• NACRES: NA.47

Properties

• Quality Level: 200
• assay: ≥97% (HPLC)
• form: powder
• mp: 175-177 °C (lit.)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: NC1=C(N)C=CC(C2=CC(N)=C(N)C=C2)=C1
• InChI: 1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2
• InChI key: HSTOKWSFWGCZMH-UHFFFAOYSA-N

Description

Application

3,3′-Diaminobenzidine has been used as a chromogen for the visualization in immunohistochemistry of chondrosarcoma sections, mammary carcinomas and brain tissue sections.

Peroxidase substrate; reagent for spectrophotometric determination of selenium.

Packaging

1, 5 g in glass bottle

10, 25 g in poly bottle

Biochem/physiol Actions

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures.

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H302 - H319 - H341 - H350
• Precautionary Statements: P201 - P301 + P312 + P330 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
• Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves