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D9564

Sigma-Aldrich

DAPI, dilactate

≥98% (HPLC)

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Synonym(s):
4′,6-Diamidino-2-phenyindole, dilactate
Empirical Formula (Hill Notation):
C16H15N5 · 2C3H6O3
Molecular Weight:
457.48
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥98% (HPLC)

form

powder

color

yellow

solubility

H2O: 20 mg/mL

ε (extinction coefficient)

28.00-32.50 at 345-351 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)

InChI key

LCAUPANIXBMNIA-UHFFFAOYSA-N

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This Item
F6057D9542MBD0020
vibrant-m

D9564

DAPI, dilactate

vibrant-m

F6057

Fluoroshield with DAPI

vibrant-m

D9542

DAPI

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

200

form

powder

form

liquid

form

powder

form

liquid

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

assay

≥98% (HPLC)

assay

-

assay

≥98% (HPLC)

assay

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

General description

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Biochem/physiol Actions

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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