D9805

Sigma-Aldrich

Fast Blue B Salt

Dye content ~95 %

Synonym(s):
Azoic Diazo No. 48, Diazo Blue B, DBB, o-Dianisidine bis(diazotized) zinc double salt, Naphthanil Diazo Blue B
Linear Formula:
C14H12N4O2Cl2 · ZnCl2
CAS Number:
Molecular Weight:
475.47
Colour Index Number:
37235
Beilstein/REAXYS Number:
1499067
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47
Pricing and availability is not currently available.

Quality Level

description

Technical grade

form

powder

composition

Dye content, ~95%

application(s)

hematology: suitable
histology: suitable

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

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Application

  • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
  • It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
  • It has also been used for the determination of the lipase activity.

Packaging

10, 100 g in glass bottle

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Hazard Codes

T

Risk Statement

45

Safety Statement

53-45

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Partial characterization of pyloric-duodenal lipase of gilthead seabream (Sparus aurata).
Nolasco H, et al.
Fish Physiology and Biochemistry, 37, 43-43 (2011)
null
A TLC bioautographic method for the detection of alpha- and beta-glucosidase inhibitors in plant extracts.
Sim?es-Pires CA, et al.
Phytochemical Analysis, 20, 511-511 (2009)
Noriyuki Osada
American journal of botany, 104(4), 550-558 (2017-04-21)
Timing of budburst ( Clear differences in the trait relationships between deciduous and evergreen broad-leaved species might be caused by different selection pressures on
Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water.
Sartory DP, et al.
Letters in Applied Microbiology, 42, 418-418 (2006)

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