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D9805

Sigma-Aldrich

Fast Blue B Salt

Dye content ~95 %, Powder

Synonym(s):

o-Dianisidine bis(diazotized) zinc double salt, Azoic Diazo No. 48, DBB, Diazo Blue B, Naphthanil Diazo Blue B

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About This Item

Linear Formula:
C14H12N4O2Cl2 · ZnCl2
CAS Number:
Molecular Weight:
475.47
Colour Index Number:
37235
Beilstein/REAXYS Number:
1499067
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Fast Blue B Salt, Dye content ~95 %

description

Technical grade

Quality Level

form

powder

composition

Dye content, ~95%

color

yellow to dark brown

mp

>300 °C (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.COc1cc(ccc1[N+]#N)-c2ccc([N+]#N)c(OC)c2

InChI

1S/C14H12N4O2.4ClH.Zn/c1-19-13-7-9(3-5-11(13)17-15)10-4-6-12(18-16)14(8-10)20-2;;;;;/h3-8H,1-2H3;4*1H;/q+2;;;;;+2/p-4

InChI key

GPPKNJIWDULNQH-UHFFFAOYSA-J

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Application

  • Fast Blue B has been used in the acid phosphatase test in Clostridium perfringens.
  • It has been used as a spraying agent in thin layer chromatography for the identification of separated components.
  • It has also been used for the determination of the lipase activity.

Biochem/physiol Actions

Fast blue B is used for the determination of acetylcholinesterase activity. Acetylcholinesterase converts 1-naphthyl acetate to 1-naphthol, which reacts with fast blue B to form a purple-coloured diazonium dye. Similarly, fast blue B is also used for α- and β-glucosidase activity.

pictograms

FlameHealth hazard

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B - Self-react. C

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C N Kennett et al.
Journal of clinical periodontology, 24(6), 424-431 (1997-06-01)
Gingival crevicular fluid (GCF) was collected from chronic periodontitis patients using plastic micropipettes and coverslip smears stained with antibodies for leukocyte markers and Toluidine Blue for mast cells. The smears consisted of 70-80% granulocytes, 10-20% monocytes/macrophages, 5% mast cells and
Overcoming the bell-shaped dose-response of cannabidiol by using Cannabis extract enriched in cannabidiol.
Gallily R, et al.
Pharmacology & Pharmacy, 6, 75-75 (2015)
Dong Ho Kang et al.
Polymers, 12(11) (2020-11-18)
In this study, composite anion exchange membranes (AEMs) were synthesized by cross-linking poly(phenylene oxide) (PPO) with cellulose functionalized by 1,4-diazabicyclo[2.2.2]-octane (DABCO) or di-guanidine (DG). The structural and morphological characteristics of the synthesized AEMs were characterized by FTIR, 1H-NMR, SEM, TEM
A TLC bioautographic method for the detection of alpha- and beta-glucosidase inhibitors in plant extracts.
Sim?es-Pires CA, et al.
Phytochemical Analysis, 20, 511-511 (2009)
Evaluation of acid phosphatase as a confirmation test for Clostridium perfringens isolated from water.
Sartory DP, et al.
Letters in Applied Microbiology, 42, 418-418 (2006)

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