MilliporeSigma
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DYNA002

Sigma-Aldrich

DyNAbind® 10 Million Compound DNA-Encoded Library

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Synonym(s):
DNA encoded library, DNA library

form

pellets

storage temp.

−20°C

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LIBHV1SEQXEOAMLS
storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C

General description

  • An off-the-shelf kit containing 2 or 5 vials
  • Each vial is for testing a single condition
  • A detailed user guide with an easy to follow protocol for using the kit
  • Analysis instructions for using the web portal to analyze the nextgen sequencing data

Application

This is a DNA-encoded library of 10 million small molecule compounds created by DyNAbind that can be used for drug discovery. Each compound is created using combinatorial chemistry to form trimers with over 200 building blocks at each of three positions. Each building block is tagged with a unique DNA barcode. When the building blocks are connected through amide bonds, the DNA is also combined forming a double-stranded DNA barcode for each compound. The compound is attached through an amide bond to a C6 linker which is then attached to an oxygen in the 5′ phosphate on the DNA.

All the compounds fit in a single microcentrifuge tube. The compounds can be incubated with a target of interest that is attached to beads. After washing, the compounds bound to the target can be detected. The DNA barcodes are amplified using PCR and then detected using nextgen sequencing. The sequencing data can be loaded to a secure portal on our website for analysis. This will generate a report of the highest affinity compounds that were bound to the target.

Several methods are used to QC the library. The initial single building blocks with their attached DNA barcodes are tested by LC-MS. PCR is used to monitor library assembly, and at completion the library is tested by nextgen sequencing to ensure reasonable compound distribution. The cost of the library includes generating a list of 50 hits or up to 60 hits if there are ties. All the hit structures will be revealed at no additional charge. There are also no additional licensing fees for using the compounds for research and development or manufacturing. This kit saves time since it can be bought and used without the negotiation required for licensing other DEL technologies.

Analysis Portal

We also provide an extended data package (DELDATA) for researchers involved in Artificial Intelligence and Machine Learning for Drug Discovery.

More information about DyNAbind

Features and Benefits

  • 10 Million compound, off-the-shelf, affordable DEL
  • Designed by DyNAbind
  • Fits in a single microcentrifuge tube
  • No sophisticated robotics required to use the kit
  • Rapid assay by binding, washing, PCR, and nextgen sequencing
  • Free analysis on our website portal
  • Structures are revealed for 50 hits or up to 60 with ties at no additional charge
  • The label license allows any use of hits in research and development or manufacturing at no additional charge

Packaging

  • 2 or 5 tubes
  • Each tube contains a single lyophilized pellet with 0.3 nmol of the library. The size of the library may vary by batch but will contain at least 10 million compounds.
  • The concentration is optimized so that each tube can be used for a single condition. For example, with two tubes, this could be used to test binding to a target and to test a negative control using the beads without a target.

Legal Information

Product of DyNAbind GmbH

Label License: For R&D use only. Not for drug, household, or other uses. There are no restrictions, additional agreements, or fees required from Merck KGaA or from DyNAbind for using the identified compounds for any purpose.
DyNAbind is a registered trademark of DyNAbind GmbH

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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T1503
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25G
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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Articles

Fragment-Based DNA-Encoded Library for Drug Discovery

Obtain over 30-fold increase in signal-to-noise ratios for better results. The DyNAbind library architecture permits dynamic reshuffling until the members are stabilized by binding to the target protein.

Future of Drug Discovery with DNA Encoded Libraries

DNA encoded libraries (DELs) offer a paradigm shift in drug discovery by leveraging molecular biology and high-throughput screening. DELs offer a full high-throughput screening library in one vial. Along with our DEL data package, machine learning and AI can be utilized to determine even more information on targets.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service