E1903

Ethidium homodimer I solution

≥95% purity (HPLC), liquid

• Synonym(s): EtDi, EthD-1, Phenanthridinium, 5,5′-[1,2-ethanediylbis(imino-3,1-propanediyl)]bis(3, 8-diamino-6-phenyl)-,dichloride,dihydrochloride
• Empirical Formula (Hill Notation): C₄₆H₅₀Cl₄N₈
• Molecular Weight: 856.75
• NACRES: NA.47
• PubChem Substance ID: 24894444
• UNSPSC Code: 12171500
• EC Number: 200-664-3
• MDL number: MFCD00210948

Properties

• Product Name: Ethidium homodimer I solution, ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO
• InChI key: ZKAJUKABUUJRLB-UHFFFAOYSA-N
• InChI: 1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
• SMILES string: Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
• assay: ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)
• form: liquid
• concentration: 2 mM in DMSO
• technique(s): titration: suitable
• color: red
• ε (extinction coefficient): ≥9000 at 490-496 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• Quality Level: 100

Description

Application

Ethidium homodimer I solution has been used to check membrane integrity in sperms.[1]

Stains ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA structures.

Used to detect DNA in solution.

Biochem/physiol Actions

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.[1]

One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: 185.0 °F - closed cup
• flash_point_c: 85 °C - closed cup
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)