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E2028

Ethidium bromide monoazide

≥95% purity (HPLC), solid

Synonym(s):

3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide, Ethidium monoazide bromide

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E2028-5MG

$447.00

$447.00

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About This Item

Empirical Formula (Hill Notation):
C21H18BrN5
CAS Number:
58880-05-0
Molecular Weight:
420.31
UNSPSC Code:
12171500
PubChem Substance ID:
329799025
NACRES:
NA.47
MDL number:
MFCD01708677

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Product Name

Ethidium bromide monoazide, ≥95% (HPLC), solid

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(ccc3c4ccc(N)cc14)N=[N+]=[N-]

InChI

1S/C21H17N5.BrH/c1-2-26-20-12-15(22)8-10-18(20)17-11-9-16(24-25-23)13-19(17)21(26)14-6-4-3-5-7-14;/h3-13,22H,2H2,1H3;1H

InChI key

GHUXAYLZEGLXDA-UHFFFAOYSA-N

assay

≥95% (HPLC)

form

solid

technique(s)

titration: suitable

color

orange

solubility

DMF: soluble, ethanol: soluble

ε (extinction coefficient)

≥5200 at 459-465 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

Quality Level

100

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This Item
E8751A784746067
Ethidium bromide monoazide ≥95% (HPLC), solid

Sigma-Aldrich

E2028

Ethidium bromide monoazide

Ethidium bromide ~95% (HPLC)

Sigma-Aldrich

E8751

Ethidium bromide

Acridine Orange 10-nonyl bromide

Sigma-Aldrich

A7847

Acridine Orange 10-nonyl bromide

Ethidium bromide solution suitable for fluorescence, ~1% in H2O

Sigma-Aldrich

46067

Ethidium bromide solution

technique(s)

titration: suitable

technique(s)

-

technique(s)

-

technique(s)

-

color

orange

color

-

color

orange to red

color

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

form

solid

form

-

form

powder or solid

form

-

solubility

DMF: soluble, ethanol: soluble

solubility

-

solubility

ethanol, DMSO: soluble

solubility

-

assay

≥95% (HPLC)

assay

~95% (HPLC)

assay

≥90% (HPLC)

assay

-

Biochem/physiol Actions

Photoactive stain covalently binds to nucleic acids in solution and in cells with damaged membranes.
Used to footprint drug binding sites on DNA, detect non-viable cells, identify ethidium binding sites on DNA and tRNA, and selectively inactivate gene expression.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ6424
MKCX9688
MKCV5954
MKCR3520
MKCQ2256

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Ji-Min Ju et al.
Nature communications, 9(1), 225-225 (2018-01-18)
Whether hematopoietic cell-restricted distribution of antigens affects the degree of thymic negative selection has not been investigated in detail. Here, we show that T cells specific for hematopoietic cell-restricted antigens (HRA) are not completely deleted in the thymus, using the
Sara Daniel et al.
Clinical epigenetics, 10(1), 138-138 (2018-11-08)
Cancer treatments have substantially improved childhood cancer survival but are accompanied by long-term complications, notably chronic inflammatory diseases. We hypothesize that cancer treatments could lead to long-term epigenetic changes in immune cells, resulting in increased prevalence of inflammatory diseases in
C-W Chang et al.
Indoor air, 21(4), 291-299 (2011-01-05)
Legionella pneumophila, aerosolized from numerous indoor facilities (e.g., shower heads, hot tubs, spas), may cause Pontiac fever (PF) and lethal pneumonia named Legionnaires' disease (LD) in humans. Reliable methods on quantitative exposure assessment of this bioaerosol are essential for the
G Loozen et al.
Molecular oral microbiology, 26(4), 253-261 (2011-07-07)
DNA-based methodology for the identification and detection of specific bacteria in dental plaque offers advantages over culturing techniques. One drawback of current molecular techniques like real-time quantitative polymerase chain reaction (RT-QPCR) is that they are not able to distinguish between
Maia Inoue et al.
Nature immunology, 19(11), 1265-1276 (2018-10-17)
The methylation of arginine residues in proteins is a post-translational modification that contributes to a wide range of biological processes. Many cytokines involved in T cell development and activation utilize the common cytokine receptor γ-chain (γc) and the kinase JAK3
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