E9379

Sigma-Aldrich

Stains-All

~95%

Synonym(s):
1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
Empirical Formula (Hill Notation):
C30H27BrN2S2
CAS Number:
Molecular Weight:
559.58
Beilstein/REAXYS Number:
3865213
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

~95%

form

powder

application(s)

hematology: suitable
histology: suitable

solubility

chloroform: 10 mg/mL

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

2-8°C

SMILES string

[Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC

InChI

1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1

InChI key

MPBRYMWMMKKRGC-UHFFFAOYSA-M

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Application

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Packaging

1, 5 g in glass bottle

Biochem/physiol Actions

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca2+ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Caution

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Chromatin and Chromosomal Protein Research III (1978)
Rehm H
Protein Biochemistry and Proteomics. The experimenter series (2006)
Kinetics of duplex formation for individual DNA strands within a single protein nanopore.
Howorka S, et al.
Proceedings of the National Academy of Sciences of the USA, 98, 12996-12996 (2001)
A comparison of the sensitivity, specificity, and molecular weight accuracy of three different commercially available Hyaluronan ELISA-like assays.
Haserodt S, et al.
Glycobiology, 21, 175-175 (2011)
Primary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix.
Lauer ME, et al.
The Journal of Biological Chemistry, 284, 5299-5299 (2009)

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