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F0888

Sigma-Aldrich

Fura 2-AM

≥95% (HPLC)

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Synonym(s):
Fura-2 pentakis(acetoxymethyl) ester
Empirical Formula (Hill Notation):
C44H47N3O24
CAS Number:
Molecular Weight:
1001.85
Beilstein/REAXYS Number:
8183748
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

assay

≥95% (HPLC)

form

powder

solubility

acetone: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc3cc(oc3cc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c4ncc(o4)C(=O)OCOC(C)=O

InChI

1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3

InChI key

VPSRLGDRGCKUTK-UHFFFAOYSA-N

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This Item
P563147989344905
Fura 2-AM ≥95% (HPLC)

F0888

Fura 2-AM

o-Cresolphthalein Complexone powder

P5631

o-Cresolphthalein Complexone

Fura 2-AM BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

47989

Fura 2-AM

FURA 2/AM Cell-permeable ester form of FURA 2.

344905

FURA 2/AM

solubility

acetone: 10 mg/mL

solubility

methanol: 1 mg/mL

solubility

DMSO: soluble

solubility

-

form

powder

form

powder

form

solid

form

solid

Quality Level

100

Quality Level

200

Quality Level

-

Quality Level

100

assay

≥95% (HPLC)

assay

-

assay

≥95.0% (HPLC)

assay

≥95% (HPLC)

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

General description

Fura 2-AM (Fura 2) is a calcium-specific ratio-metric fluorescent dye. It is also known as acetoxy-methyl-ester. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505nm, and the excitation peak ranges between 340-380nm and is the preferred calcium indicator.

Application

Fura 2-AM is a popular probe used to determine dynamic changes in cytosolic free calcium in intact living cells.

Features and Benefits

The major benefits of using the ester form of Fura 2 as Fura 2-AM are: It is cell-permeable. It can be loaded into cells easily. Another advantage of using Fura 2-AM over single wavelength dyes is that the ratio signal is independent of : The dye concentration. Illumination intensity, and Optical path length.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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