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F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

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CAS Number:
26873-85-8
MDL number:
MFCD00081599
NACRES:
NA.75

sterility

0.2 μm filtered

Quality Level

200

type

Type 400

concentration

20% in H2O

application(s)

hematology
histology

storage temp.

2-8°C

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1 of 4

This Item
F2637GE17-0300-50F2878
Sigma-Aldrich

Sigma-Aldrich

F5415

Ficoll® solution

Sigma-Aldrich

Sigma-Aldrich

F2637

Ficoll® 400

Ficoll® PM 400 Cytiva 17-0300-50, pack of 500 g

GE17-0300-50

Ficoll® PM 400

Sigma-Aldrich

Sigma-Aldrich

F2878

Ficoll® PM 70

application(s)

hematology
histology

application(s)

-

application(s)

-

application(s)

-

storage temp.

2-8°C

storage temp.

room temp

storage temp.

-

storage temp.

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

200

sterility

0.2 μm filtered

sterility

-

sterility

-

sterility

-

type

Type 400

type

-

type

-

type

Type 70

General description

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Application

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells
  • Isolation of CD133 negative cells
  • Isolation and expansion of circulating endothelial progenitor cells.

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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-
25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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H8889

Histopaque®-1077 Hybri-Max™

Junchao Xing et al.
Tissue engineering. Part C, Methods, 25(3), 137-147 (2019-02-09)
Patient-specific individual tissue-engineered bones (iTEBs) have been recognized as a promising strategy for treating large bone defects. However, current construction protocols of iTEBs vary between lots and lack standardization and quality control, hampering further research and application. This study was
Effect of mononuclear cells on morphometric of kidney parameters in hyperglycemic rat.
Salmani F, et al.
Annals of Experimental Biology, 2(3), 1-7 (2014)
Analysis of Structural and Functional Glomerular Changes in Hyperglycemic Rats that Treated with Human Umbilical Cord Blood Derived CD133 Negative Ccells.
Feizi F, et al.
Physical Chemistry, 1(1) (2014)
Circulation enrichment of functional endothelial progenitor cells by infantile phototherapy.
Siavashi V, et al.
Journal of Cellular Biochemistry, 118(2), 330-340 (2017)
Juliane Schmidt et al.
PloS one, 6(3), e18184-e18184 (2011-03-30)
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have
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Protocols

Hematopoietic Stem Cell Culture Protocols

Step-by-step hematopoietic stem cell culture protocols for isolation, expansion and differentiation of CD34+ hematopoietic progenitor cells including CFU assays. It can be cultured under defined conditions designed either to promote self-renewal and increase the number of primitive cells.

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