F5415

Ficoll^® solution

Type 400, 20% in H₂O

• Synonym(s): Ready-to-use density gradient media
• CAS Number: 26873-85-8
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00081599

Properties

• sterility: 0.2 μm filtered
• Quality Segment: 200
• type: Type 400
• form: liquid
• concentration: 20% in H₂O
• technique(s): titration: suitable
• color: clear colorless
• solubility: water: soluble
• density: 1.055-1.075 g/mL
• application(s): hematology; histology
• storage temp.: 2-8°C

Description

General description

Ficoll^® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll^® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.

Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Application

Ficoll^® has been used in

• Isolation of cord blood mononuclear cells
• Isolation of CD133 negative cells
• Isolation and expansion of circulating endothelial progenitor cells.

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable