F6797

Millipore

Frey mycoplasma broth base

suitable for microbiology, NutriSelect® Plus

NACRES:
NA.85
Pricing and availability is not currently available.

form

powder

Quality Level

shelf life

limited shelf life, expiry date on the label

mfr. no.

NutriSelect® Plus

application(s)

microbiological culture: suitable
microbiology: suitable

final pH

7.7±0.2 (25 °C)

Featured Industry

Clinical Testing
Food and Beverages
Life Science and Biopharma
Veterinary

suitability

mycoplasma

Application

Used for cultivation of Avian Mycoplasma.
Frey mycoplasma broth base may be used as a culture medium for examining various proteins of Mycoplasma pulmonis.

Components

Ingredients (g/L)
Casein enzymic hydrolysate, 7.50
Papaic digest of soyabean meal, 2.50
Yeast extract, 5.00
Sodium chloride, 5.00
Potassium chloride, 0.40
Magnesium sulphate, 0.20
Disodium phosphate, 1.60
Monopotassium phosphate, 0.10

Preparation Note

Suspend 22.3 g of Frey Mycoplasma Broth Base in 900 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 100 ml of sterile heat inactivated horse serum (H1138). Mix well before dispensing.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Certificate of Analysis
Certificate of Origin
Protein variability among strains of Mycoplasma pulmonis.
Watson HL, et al.
Infection and Immunity, 55(11), 2838-2840 (1987)
Articles
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Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
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