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G5882

Sigma-Aldrich

Glutaraldehyde solution

Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative

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Synonym(s):
Glutaric dialdehyde solution, Pentane-1,5-dial
Linear Formula:
OHC(CH2)3CHO
CAS Number:
Molecular Weight:
100.12
Beilstein/REAXYS Number:
605390
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

type

Grade I

form

liquid

concentration

25% in H2O

technique(s)

electron microscopy: suitable (fixative)
electron microscopy: suitable

color

colorless

useful pH range

2.9

mp

-10 °C ((14 °F ))

solubility

water: soluble at 20 °C

application(s)

cell analysis

shipped in

dry ice

storage temp.

−20°C

SMILES string

[H]C(CCCC([H])=O)=O

InChI

1S/C5H8O2/c6-4-2-1-3-5-7/h4-5H,1-3H2

InChI key

SXRSQZLOMIGNAQ-UHFFFAOYSA-N

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This Item
G7651G7526340855
Glutaraldehyde solution Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative

G5882

Glutaraldehyde solution

Glutaraldehyde solution Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use

G7651

Glutaraldehyde solution

Glutaraldehyde solution Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use

G7526

Glutaraldehyde solution

Glutaraldehyde solution 50 wt. % in H2O

340855

Glutaraldehyde solution

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

mp

-10 °C ((14 °F ))

mp

-21  °C ((-6 °F))

mp

-

mp

-21  °C ((-6 °F))

form

liquid

form

liquid

form

liquid

form

liquid

type

Grade I

type

Grade I

type

Grade I

type

-

General description

Glutaraldehyde (Glutaric dialdehyde, Pentane-1,5-dial) is an aliphatic dialdehyde widely utilized as a versatile biochemical reagent. Glutaraldehyde solutions contain both monomers and polymers with the difference in their fixation properties. Its applications include serving as a protein crosslinker, facilitating enzyme immobilization in microscopy, histochemistry, and cytochemistry. This compound acts as an amine-reactive homobifunctional crosslinker and a cell fixative before SDS-PAGE, staining, or electron microscopy, making it suitable for diverse morphological studies.
Glutaraldehyde efficiently crosslinks amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, it has been used in certain reactions to directly couple biotin hydrazides to nucleic acids, suggesting potential applications in conjugating fluorescent hydrazides and hydroxylamines to DNA. In histology studies, Glutaraldehyde is employed to preserve tissue sections, preparing them for closer examination.

Application

Glutaraldehyde solution has been used:
  • to fix the blood samples for scanning electron microscopy (SEM)
  • to fix scaffold pieces for SEM analysis
  • in drug treatments to treat K562 (leukemia) cells to permanently cross-link cell components

Biochem/physiol Actions

Glutaraldehyde can be used in biomedical research to fix cells. It can also be used as a tool to investigate healthy and pathologic red blood cells. Medical equipment like spirometry tubing, endoscopes, dialyzers, hemodialysis proportioning and dialysate delivery systems, endocavitary probes, transducers, anesthesia and respiratory therapy equipment, and reuse of laparoscopic disposable plastic trocars can be disinfected with glutaraldehyde.

Features and Benefits

  • Versatile and adaptable for wide variety of research applications
  • Ready-made solution reduces the need for preparation time

Other Notes

For additional information on our range of Biochemicals, please complete this form.
Recommended for techniques demanding high purity.
To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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