The solution is created with deionized water. It would be best to dilute by a factor of 25 in a deionized water which has a low absorbance value to ensure that the material remains high purity and with low coloration.
G5882
Glutaraldehyde solution
Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Synonym(s):
Glutaric dialdehyde solution, Pentane-1,5-dial
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 x 1 mL | Estimated to ship TODAYfromSAINT LOUIS | $192.00 | |
| 50 mL | Estimated to ship TODAYfromSAINT LOUIS | $216.00 | |
| 80 mL | Estimated to ship TODAYfromSAINT LOUIS | $261.00 | |
| 10 x 10 mL | Estimated to ship TODAYfromSAINT LOUIS | $278.00 | |
| 100 mL | Estimated to ship TODAYfromSAINT LOUIS | $292.00 | |
| 1 L | Estimated to ship onMay 18, 2026fromSAINT LOUIS | $2,110.00 |
About This Item
Linear Formula:
OHC(CH2)3CHO
CAS Number:
Molecular Weight:
100.12
UNSPSC Code:
12352114
NACRES:
NA.21
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
605390
Form:
liquid
Quality Level
type
Grade I
form
liquid
concentration
25% in H2O
technique(s)
electron microscopy: suitable (fixative), electron microscopy: suitable
color
colorless
useful pH range
2.9
mp
-10 °C ((14 °F ))
solubility
water: soluble at 20 °C
application(s)
cell analysis
shipped in
dry ice
storage temp.
−20°C
SMILES string
[H]C(CCCC([H])=O)=O
InChI
1S/C5H8O2/c6-4-2-1-3-5-7/h4-5H,1-3H2
InChI key
SXRSQZLOMIGNAQ-UHFFFAOYSA-N
General description
Glutaraldehyde (Glutaric dialdehyde, Pentane-1,5-dial) is an aliphatic dialdehyde widely utilized as a versatile biochemical reagent. Glutaraldehyde solutions contain both monomers and polymers with the difference in their fixation properties. Its applications include serving as a protein crosslinker, facilitating enzyme immobilization in microscopy, histochemistry, and cytochemistry. This compound acts as an amine-reactive homobifunctional crosslinker and a cell fixative before SDS-PAGE, staining, or electron microscopy, making it suitable for diverse morphological studies.
Glutaraldehyde efficiently crosslinks amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, it has been used in certain reactions to directly couple biotin hydrazides to nucleic acids, suggesting potential applications in conjugating fluorescent hydrazides and hydroxylamines to DNA. In histology studies, Glutaraldehyde is employed to preserve tissue sections, preparing them for closer examination.
Glutaraldehyde efficiently crosslinks amine and hydrazine derivatives to proteins and other amine-containing polymers. Notably, it has been used in certain reactions to directly couple biotin hydrazides to nucleic acids, suggesting potential applications in conjugating fluorescent hydrazides and hydroxylamines to DNA. In histology studies, Glutaraldehyde is employed to preserve tissue sections, preparing them for closer examination.
Application
Glutaraldehyde solution has been used:
- to fix the blood samples for scanning electron microscopy (SEM)
- to fix scaffold pieces for SEM analysis
- in drug treatments to treat K562 (leukemia) cells to permanently cross-link cell components
Biochem/physiol Actions
Glutaraldehyde can be used in biomedical research to fix cells. It can also be used as a tool to investigate healthy and pathologic red blood cells. Medical equipment like spirometry tubing, endoscopes, dialyzers, hemodialysis proportioning and dialysate delivery systems, endocavitary probes, transducers, anesthesia and respiratory therapy equipment, and reuse of laparoscopic disposable plastic trocars can be disinfected with glutaraldehyde.
Features and Benefits
- Versatile and adaptable for wide variety of research applications
- Ready-made solution reduces the need for preparation time
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Recommended for techniques demanding high purity.
To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.
1 of 1
This Item | |||
|---|---|---|---|
| solubility water: soluble at 20 °C | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C |
| form liquid | form liquid | form liquid | form liquid |
| mp -10 °C ((14 °F )) | mp - | mp -21 °C ((-6 °F)) | mp -21 °C ((-6 °F)) |
| useful pH range 2.9 | useful pH range - | useful pH range - | useful pH range - |
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Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
8A - Combustible corrosive hazardous materials
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
supp_hazards
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| G5882-10X10ML | 04061838255723 |
| G5882-50ML | 04061838255754 |
| G5882-1L | 04061838255747 |
| G5882-100ML | 04061838255716 |
| G5882-10X1ML | 04061838255730 |
| G5882-80ML | 04061833638583 |
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How can I dilute 25% solution to make it 1% gluteraldyhyde solution? What should I dilute it in? Is it just water or id there a particular buffer that is recommended?
1 answer-
Helpful?
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What is the best way to thaw the glutaraldehyde solution if it has been stored at -20°C?
1 answer-
The glutaraldehyde solution can be moved from the -20°C freezer to a refrigerator (2-8°C) for gradual thawing. This method ensures that the solution thaws evenly and minimizes the risk of degradation. To maintain the integrity of the glutaraldehyde solution, avoid refreezing it once thawed.
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Hi, I have a question about glutaraldehyde storage. After opening the bottle, should I continue to store it at -20°C? I’m concerned because I may need to freeze and thaw it multiple times. Is this a problem, or is there a better way to store and use it
1 answer-
This product is tested for suitability in electron microscopy. In order to prevent degradation over time, the material should be stored at -20°C throughout the shelf life of the product. The solution may be transferred to smaller vials to minimize repeated freeze/thaw cycles.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is this concentration of 25% expressed in terms of weight by weight (w/w), weight by volume (w/v), or volume by volume (v/v)?
1 answer-
The concentration of the product is 25% w/w solution.
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