H23830

HEPES

≥99% (titration), crystalline, reagent grade

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• UNSPSC Code: 12161700
• NACRES: NA.25
• MDL number: MFCD00006158
• Beilstein/REAXYS Number: 883043

Properties

• Product Name: HEPES, reagent grade, ≥99% (titration), crystalline
• biological source: synthetic
• Quality Segment: 200
• grade: reagent grade
• assay: ≥99% (titration)
• form: crystalline
• storage condition: (Tightly closed. Dry)
• impurities: ≤1% water
• color: white
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• mp: 212.6 °C
• solubility: water: 1 M, clear, colorless
• density: 1.44 g/cm³ at 20 °C
• cation traces: Fe: <5 ppm
• storage temp.: room temp
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, serves as a zwitterionic N-substituted aminosulfonic acid buffer with exceptional buffering capacity (pH 6.8-8.2, pKa 7.48 at 25°C). Recognized for versatility, it is extensively employed in cell biology, biochemical, and biological research. Its noteworthy feature lies in its neutrality towards metal ions, making it a preferred "non-coordinating buffer" suitable for solutions containing metals without affecting their activity. In cell biology studies, HEPES excels at maintaining stable physiological pH despite carbon dioxide fluctuations. It outperforms bicarbonate buffers commonly used in this context.

HEPES extends its utility as a preferred separator in creating pH gradients for isoelectric focusing, a technique in protein separation. Beyond these applications, it proves beneficial in immunoprecipitation, cell lysis, live cell imaging, establishing its indispensability across diverse research areas due to its versatility and minimal interaction with other molecules.

Application

HEPES has been used:

• as a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
• as a component of Hanks′ solution used in the degradation testing of Iron
• as a component of HEPES medium in the transfection of cell culture with plasmids
• along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
• as a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies

Features and Benefits

• High purity product for biochemical and biological research
• Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C

• Broad buffer range between pH 6.8 to 8.2
• Zwitterionic at biological pH
• High solubility and stability
• Low UV and visible light spectrum absorbance
• Negligible metal ion binding
• Suitable for cell culture, electrophoresis, in vitro methods, etc.

Other Notes

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Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable