This product contains 1 mg each of ribonuclease A, cytochrome c, holo-transferrin, and apo-myoglobin per vial. For assay purposes, reconstitute the entire vial in 4 mL of water for a 1 mg/mL concentration. A subsequent 4-fold dilution to 0.25 mg/mL may be performed and 3 µL of this solution can be injected. The contents may also be reconstituted at 1 mg/mL of 50 mM acetic acid or 5% acetonitrile containing 0.1% trifluoroacetic acid/0.1% triethylamine.
H2899
HPLC protein standard mixture
analytical standard
Synonym(s):
Protein Calibration Mix
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Check Cart for Availability | $127.00 |
About This Item
NACRES:
NA.24
UNSPSC Code:
85151701
grade
analytical standard
Quality Segment
form
solid
analyte chemical class(es)
amino acids, peptides, proteins
technique(s)
HPLC: suitable
application(s)
food and beverages
format
multi-component solution
storage temp.
−20°C
Application
HPLC protein standard mixture has been used as a standard for the separation of proteins in cell extract samples of bacteria using high performance liquid chromatography (HPLC).
Packaging
Vial contains approximately 1 mg each of ribonuclease A, cytochrome c, holo-transferrin and apomyoglobin.
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This Item | |||
|---|---|---|---|
| format multi-component solution | format multi-component solution | format multi-component solution | format single component solution |
| form solid | form powder | form liquid | form - |
| technique(s) HPLC: suitable | technique(s) HPLC: suitable | technique(s) gel permeation chromatography (GPC): suitable | technique(s) gel permeation chromatography (GPC): suitable |
| grade analytical standard | grade analytical standard | grade analytical standard | grade analytical standard |
| analyte chemical class(es) amino acids, peptides, proteins | analyte chemical class(es) amino acids, peptides, proteins | analyte chemical class(es) amino acids, peptides, proteins | analyte chemical class(es) amino acids, peptides, proteins |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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What is the recommended reconstitution conditions for this standard material?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Could you provide an order of retention times of when each of these components elute off a protein C4 column (H2899 protein mixture)? Thank you.
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The exact method is considered proprietary. However, for a C4 column, it should elute ribonuclease A first, followed by cytochrome C, holo-transferrin, and apomyoglobin.
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What is each componant's actual molecular weight (ribonuclease A, cytochrome c, holo-transferrin and apomyoglobin)?
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The molecular weight of each component is not reported. See below for the expected values:
Ribonuclease A: approx. 13.7 kDa
Cytochrome C: approx. 12.3 kDa
holo-Transferrin: 72.1 - 78.5 kDa
Myoglobin: approx. 17.6 kDaHelpful?
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