H7126

Supelco

Vitamin B12a hydrochloride

analytical standard

Synonym(s):
Hydroxocobalamin hydrochloride
Empirical Formula (Hill Notation):
C62H89CoN13O15P · HCl
CAS Number:
Molecular Weight:
1382.82
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.24

Quality Level

grade

analytical standard

assay

≥95%

form

neat

application(s)

HPLC: suitable
gas chromatography (GC): suitable

color

dark red to brown-red

Featured Industry

Forensics and Toxicology
Veterinary
Vitamins, Nutraceuticals, and Natural Products

format

neat

storage temp.

2-8°C

SMILES string

Cl.C[C@H](CNC(=O)CC[C@]1(C)[C@@H](CC(N)=O)[C@H]2N([Co+]O)C1=C(C)C3=NC(=CC4=NC(=C(C)C5=N[C@]2(C)[C@@](C)(CC(N)=O)[C@@H]5CCC(N)=O)[C@@](C)(CC(N)=O)[C@@H]4CCC(N)=O)C(C)(C)[C@@H]3CCC(N)=O)OP([O-])(=O)O[C@H]6[C@@H](O)[C@H](O[C@@H]6CO)n7cnc8cc(C)c(C)cc78

InChI

1S/C62H90N13O14P.ClH.Co.H2O/c1-29-20-39-40(21-30(29)2)75(28-70-39)57-52(84)53(41(27-76)87-57)89-90(85,86)88-31(3)26-69-49(83)18-19-59(8)37(22-46(66)80)56-62(11)61(10,25-48(68)82)36(14-17-45(65)79)51(74-62)33(5)55-60(9,24-47(67)81)34(12-15-43(63)77)38(71-55)23-42-58(6,7)35(13-16-44(64)78)50(72-42)32(4)54(59)73-56;;;/h20-21,23,28,31,34-37,41,52-53,56-57,76,84H,12-19,22,24-27H2,1-11H3,(H15,63,64,65,66,67,68,69,71,72,73,74,77,78,79,80,81,82,83,85,86);1H;;1H2/q;;+3;/p-3/t31-,34-,35-,36-,37+,41-,52-,53-,56-,57+,59-,60+,61+,62+;;;/m1.../s1

InChI key

KEHNCSYXYMMUCO-BXSGNKEGSA-K

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General description

Vitamin B12a is an organic derivative, used as a powerful antidote for cyanide poisoning. It is a storage and delivery form of vitamin B12 and its mode of action involves the formation of non-toxic cyanocobalamin.

Application

Vitamin B12a hydrochloride has been used as an analytical standard in the analysis of hydroxocobalamin in plasma samples of pigs using liquid chromatography coupled with mass spectrometry (LC-MS/MS).
Vitamin B12a may be used as an analytical reference standard for the quantification of the analyte in pharmaceutical preparations using microbore reversed-phase HPLC with spectrophotometric, ion-spray ionization MS and inductively coupled plasma MS detection.
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Packaging

1 g in glass bottle
100 mg in glass bottle

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Simultaneous determination of hydroxocobalamin and its cyanide complex cyanocobalamin in human plasma by high-performance liquid chromatography Application to pharmacokinetic studies after high-dose hydroxocobalamin as an antidote for severe cyanide poisoning
Astier A and Baud JF
Journal of Chromatography. B, Biomedical Sciences and Applications, 667(1), 129-135 (1995)
R Pace et al.
Clinical toxicology (Philadelphia, Pa.), 52(7), 647-650 (2014-08-05)
Pre-hospital administration of hydroxocobalamin (B12a) is used for empiric treatment of cyanide poisoning because cyanide poisoning is difficult to identify and requires immediate treatment. B12a interferes with the accuracy of several blood laboratory tests. This study aimed to explore how...
Determination of cobalamins and cobinamides by microbore reversed-phase HPLC with spectrophotometric, ion-spray ionization MS and inductively coupled plasma MS detection
Chassaigne H and Lobinski R
Analytica Chimica Acta, 359(3), 227-235 (1998)
Jasmin Federizon et al.
Pharmaceutics, 13(1) (2021-01-21)
Cobalt porphyrin phospholipid (CoPoP) can incorporate within bilayers to enable non-covalent surface-display of antigens on liposomes by mixing with proteins bearing a polyhistidine tag (his-tag); however, the mechanisms for how this occurs are poorly understood. These were investigated using the...
Harvey A Schwertner et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 905, 10-16 (2012-09-04)
Cyanide poisoning occurs in individuals after fire smoke inhalation and after oral ingestion of cyanide. Hydroxocobalamin (HOCbl), a hydroxylated form of vitamin B(12), is often used as an antidote to treat cyanide toxicity. It has a high affinity for cyanide...

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