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H7126

Supelco

Vitamin B12a hydrochloride

analytical standard

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Synonym(s):
Hydroxocobalamin hydrochloride
Empirical Formula (Hill Notation):
C62H89CoN13O15P · HCl
CAS Number:
Molecular Weight:
1382.82
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.24

grade

analytical standard

Quality Level

assay

≥95%

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

color

dark red to brown-red

application(s)

forensics and toxicology
veterinary
vitamins, nutraceuticals, and natural products

format

neat

storage temp.

2-8°C

SMILES string

Cl.C[C@H](CNC(=O)CC[C@]1(C)[C@@H](CC(N)=O)[C@H]2N([Co+]O)C1=C(C)C3=NC(=CC4=NC(=C(C)C5=N[C@]2(C)[C@@](C)(CC(N)=O)[C@@H]5CCC(N)=O)[C@@](C)(CC(N)=O)[C@@H]4CCC(N)=O)C(C)(C)[C@@H]3CCC(N)=O)OP([O-])(=O)O[C@H]6[C@@H](O)[C@H](O[C@@H]6CO)n7cnc8cc(C)c(C)cc78

InChI

1S/C62H90N13O14P.ClH.Co.H2O/c1-29-20-39-40(21-30(29)2)75(28-70-39)57-52(84)53(41(27-76)87-57)89-90(85,86)88-31(3)26-69-49(83)18-19-59(8)37(22-46(66)80)56-62(11)61(10,25-48(68)82)36(14-17-45(65)79)51(74-62)33(5)55-60(9,24-47(67)81)34(12-15-43(63)77)38(71-55)23-42-58(6,7)35(13-16-44(64)78)50(72-42)32(4)54(59)73-56;;;/h20-21,23,28,31,34-37,41,52-53,56-57,76,84H,12-19,22,24-27H2,1-11H3,(H15,63,64,65,66,67,68,69,71,72,73,74,77,78,79,80,81,82,83,85,86);1H;;1H2/q;;+3;/p-3/t31-,34-,35-,36-,37+,41-,52-,53-,56-,57+,59-,60+,61+,62+;;;/m1.../s1

InChI key

KEHNCSYXYMMUCO-BXSGNKEGSA-K

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1 of 4

This Item
W332208PHR103704573
Vitamin B12a hydrochloride analytical standard

H7126

Vitamin B12a hydrochloride

Thiamine hydrochloride ≥98%, FCC, FG

W332208

Thiamine hydrochloride

Thiamine hydrochloride Pharmaceutical Secondary Standard; Certified Reference Material

PHR1037

Thiamine hydrochloride

Thiamine hydrochloride tested according to Ph. Eur.

04573

Thiamine hydrochloride

application(s)

forensics and toxicology
veterinary
vitamins, nutraceuticals, and natural products

application(s)

flavors and fragrances

application(s)

food and beverages

application(s)

-

format

neat

format

-

format

neat

format

-

Quality Level

100

Quality Level

400

Quality Level

300

Quality Level

200

grade

analytical standard

grade

FG, Halal, Kosher

grade

certified reference material, pharmaceutical secondary standard

grade

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

General description

Vitamin B12a is an organic derivative, used as a powerful antidote for cyanide poisoning. It is a storage and delivery form of vitamin B12 and its mode of action involves the formation of non-toxic cyanocobalamin.

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Vitamin B12a hydrochloride has been used as an analytical standard in the analysis of hydroxocobalamin in plasma samples of pigs using liquid chromatography coupled with mass spectrometry (LC-MS/MS).
Vitamin B12a may be used as an analytical reference standard for the quantification of the analyte in pharmaceutical preparations using microbore reversed-phase HPLC with spectrophotometric, ion-spray ionization MS and inductively coupled plasma MS detection.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Sigma-Aldrich

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Cyanocobalamin meets USP testing specifications

Sigma-Aldrich

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(+)-γ-Tocopherol analytical standard

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Vitamin B12 BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥98%

Sigma-Aldrich

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Vitamin B12

Tetrahydrofuran-d8 ≥99.5 atom % D, contains 0.03 % (v/v) TMS

Sigma-Aldrich

437727

Tetrahydrofuran-d8

Determination of cobalamins and cobinamides by microbore reversed-phase HPLC with spectrophotometric, ion-spray ionization MS and inductively coupled plasma MS detection
Chassaigne H and Lobinski R
Analytica Chimica Acta, 359(3), 227-235 (1998)
Simultaneous determination of hydroxocobalamin and its cyanide complex cyanocobalamin in human plasma by high-performance liquid chromatography Application to pharmacokinetic studies after high-dose hydroxocobalamin as an antidote for severe cyanide poisoning
Astier A and Baud JF
Journal of Chromatography. B, Biomedical Sciences and Applications, 667(1), 129-135 (1995)
R Pace et al.
Clinical toxicology (Philadelphia, Pa.), 52(7), 647-650 (2014-08-05)
Pre-hospital administration of hydroxocobalamin (B12a) is used for empiric treatment of cyanide poisoning because cyanide poisoning is difficult to identify and requires immediate treatment. B12a interferes with the accuracy of several blood laboratory tests. This study aimed to explore how
Jasmin Federizon et al.
Pharmaceutics, 13(1) (2021-01-21)
Cobalt porphyrin phospholipid (CoPoP) can incorporate within bilayers to enable non-covalent surface-display of antigens on liposomes by mixing with proteins bearing a polyhistidine tag (his-tag); however, the mechanisms for how this occurs are poorly understood. These were investigated using the
Harvey A Schwertner et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 905, 10-16 (2012-09-04)
Cyanide poisoning occurs in individuals after fire smoke inhalation and after oral ingestion of cyanide. Hydroxocobalamin (HOCbl), a hydroxylated form of vitamin B(12), is often used as an antidote to treat cyanide toxicity. It has a high affinity for cyanide

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