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I3021

Sigma-Aldrich

IGEPAL® CA-630

viscous liquid

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Synonym(s):
Octylphenoxy poly(ethyleneoxy)ethanol, branched
Linear Formula:
(C2H4O)nC14H22O
CAS Number:
NACRES:
NA.21

description

non-ionic

Quality Level

form

viscous liquid

mol wt

~603

CMC

0.08 mM (20-25°C)

viscosity

250-350 cP, neat(25 °C)(lit.)

density

1.06 g/cm3 at 25 °C (lit.)

HLB

13

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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This Item
I8896542334238589
IGEPAL® CA-630 viscous liquid

Sigma-Aldrich

I3021

IGEPAL® CA-630

IGEPAL® CA-630 for molecular biology

Sigma-Aldrich

I8896

IGEPAL® CA-630

IGEPAL® CO-630 average Mn 617

Sigma-Aldrich

542334

IGEPAL® CO-630

IGEPAL® CA-720 average Mn ~735

Sigma-Aldrich

238589

IGEPAL® CA-720

form

viscous liquid

form

liquid

form

-

form

-

mol wt

~603

mol wt

-

mol wt

average Mn 617

mol wt

average Mn ~735

CMC

0.08 mM (20-25°C)

CMC

0.08 mM (20-25°C)

CMC

-

CMC

-

viscosity

250-350 cP, neat(25 °C)(lit.)

viscosity

-

viscosity

-

viscosity

-

density

1.06 g/cm3 at 25 °C (lit.)

density

-

density

1.056 g/mL at 25 °C (lit.)

density

1.104 g/mL at 25 °C (lit.)

General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL® CA-630 has been used:
  • as a component of grinding buffer along with lithium dodecyl sulphate, tris-HCl, EDTA, NaCl, sodium deoxycholate, dithiothreitol and thiourea in RNA extraction process
  • along with Tris- HCl in incubation of Tachyzoites cultured in vero cells
  • as a component of nuclear extract buffer D+ containing HEPES, glycerol, KCl and EDTA for gel shift analysis
  • as a component of PI buffer along with NaH2PO4 to wash slides in FISH (fluorescence in situ hybridization) procedure
  • for solubilizing and isolation of membrane proteins

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Solvay

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

This page shows how to solubilize membrane proteins with products from Cytiva.

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