If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
I5500
Insulin from bovine pancreas
≥25 USP units/mg (HPLC), powder
Synonym(s):
Insulin (cattle)
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About This Item
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form
powder
Quality Level
specific activity
≥25 USP units/mg (HPLC)
impurities
≤0.5 EU/mg endotoxin
solubility
water: 2 mg/mL, clear, colorless (pH: 2)
cation traces
Zn: ≤1.0%
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
InChI key
IXIBAKNTJSCKJM-BUBXBXGNSA-N
Gene Information
cow ... INS(280829)
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1 of 4
This Item | I6634 | I1633 | I1882 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Gene Information cow ... INS(280829) | Gene Information cow ... INS(280829) | Gene Information cow ... INS(280829) | Gene Information bovine ... INS(280829) |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| solubility water: 2 mg/mL, clear, colorless (pH: 2) | solubility acetic acid: 2 mg/mL (pH: 2-3), hydrochloric acid: 2 mg/mL (pH: 2-3) | solubility - | solubility acetic acid: 2 mg/mL (pH: 2-3), hydrochloric acid: 2 mg/mL (pH: 2-3) |
Application
- Cross-frontal mode: An alternative methodology for Taylor dispersion analysis of monomodal sample.: This research presents a novel methodology for Taylor dispersion analysis, which could be applied to the study of insulin dispersion and its implications in biochemical research (Gouyon et al., 2023).
Biochem/physiol Actions
Other Notes
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Certificates of Analysis (COA)
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How can I determine the shelf life / expiration / retest date of this product?
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Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How do you determine the potency that is given in the CoA. Is the material qualified against another Insulin bovine standard (primary standard, e.g. Insulin (bovine) CRS)?
1 answer-
The potency of this product is determined by the USP HPLC method and calculated based on the HPLC area %. The material is tested against the USP human insulin standard, product 1342106.
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How should stock solutions of item I5500, Insulin from bovine pancreas be stored?
1 answer-
Stock vials of bovine insulin should be stored at -20°C in a non-frost-free freeze.
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Is Insulin product (I5500) a lyophilization powder?
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Yes, this product is lyophilized
Helpful?
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What is the amino acid sequence for insulin?
1 answer-
The sequence and structure of insulin can be found on the product information sheet (under Documents, above).
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What is the comparison between International Units (IU) and USP units for insulin?
1 answer-
The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.
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How many units of insulin are present in my vial of Product I5500, Insulin from bovine pancreas?
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Product No. I5500 has a specification of not less than 27 USP units/mg. The exact value for the product received can be found the on lot-specific Certificate of Analysis.
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How can I store the insulin solution? How long?
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The USP recommends that solutions of insulin be stored at 2-8°C. It is not recommended to freeze solutions of insulin, as it can aggregate. Sterile solutions should be stable for at least 1 year at 2-8°C.
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What can be used to make a solution of insulin?
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Insulin is soluble in dilute acids and alkalis. The solubility of insulin is tested at 2 mg/mL in water (acidified to pH 2 with hydrochloric acid). One can also use 1 mL of water acidified to pH 2 with 10 μL of glacial acetic acid to dissolve 2 mg of insulin. A 10 mg/mL solution of bovine insulin in 25 mM HEPES at pH 8.2 is offered as Product No. I0516.
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