I6758

IPTG

≥99% (TLC), ≤0.1% Dioxane

• Synonym(s): Isopropyl β-D-1-thiogalactopyranoside, IPTG, Isopropyl β-D-thiogalactoside
• Empirical Formula (Hill Notation): C₉H₁₈O₅S
• CAS Number: 367-93-1
• Molecular Weight: 238.30
• Beilstein: 4631
• EC Number: 206-703-0
• MDL number: MFCD00063273
• PubChem Substance ID: 24896093
• NACRES: NA.31

Properties

• biological source: synthetic
• Quality Level: 300
• description: non-ionic
• Assay: ≥99% (TLC)
• form: powder
• technique(s): thin layer chromatography (TLC): suitable
• impurities: ≤0.1% Dioxane
• storage temp.: 2-8°C
• SMILES string: CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• InChI key: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Description

Application

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Other Notes

Non-metabolizable galactose analog.

Reconstitution

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H350
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves