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I8377

Sigma-Aldrich

Iodonitrotetrazolium chloride

Used in colorimetric assays.

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Synonym(s):
2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT
Empirical Formula (Hill Notation):
C19H13ClIN5O2
CAS Number:
Molecular Weight:
505.70
Beilstein:
4093224
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

Assay

≥98% (TLC)

form

powder

mp

240 °C (dec.) (lit.)

solubility

H2O: 4 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

InChI key

JORABGDXCIBAFL-UHFFFAOYSA-M

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1 of 4

This Item
58030I10406T0138
Iodonitrotetrazolium chloride BioReagent, ≥97.0% (calc. on dry substance, NT)

Sigma-Aldrich

58030

Iodonitrotetrazolium chloride

Essential+ Grade
Tetrazolium Violet

Sigma-Aldrich

T0138

Tetrazolium Violet

-
form

powder

form

-

form

-

form

powder

mp

240 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.), ~245 °C (dec.)

mp

240 °C (dec.) (lit.)

mp

247-249 °C

solubility

H2O: 4 mg/mL

solubility

methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)

solubility

-

solubility

methanol: 1 mg/mL, clear

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.
Electron acceptor for the colorimetric assay of various dehydrogenases

related product

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Flam. Sol. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 3


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Ignatoski KMW and Ethier SP
Breast Cancer Research and Treatment, 54, 173-173 (1999)
F Mermillod-Blondin et al.
Water research, 39(9), 1687-1698 (2005-05-19)
Biogeochemical processes mediated by microorganisms in river sediments (hyporheic sediments) play a key role in river metabolism. Because biogeochemical reactions in the hyporheic zone are often limited to the top few decimetres of sediments below the water-sediment interface, slow filtration
T J Mankelow et al.
The Biochemical journal, 358(Pt 2), 315-324 (2001-08-22)
Diethyl pyrocarbonate (DEPC), a histidine-modifying reagent, has been utilized to demonstrate the importance of histidine residues in the functioning of proteins. In previous studies of the NADPH oxidase, histidine residues have been determined to be important in the ability of
Xiao-mei Xie et al.
Journal of environmental sciences (China), 16(2), 252-255 (2004-05-13)
The impact of triazophos, bensulfuron-methyl, chlobenthiazone on soil biochemical characteristics in a paddy soil under controlled moisture (flooded soil) and temperature (25 degrees C) condition was studied. The electron transport system (ETS)/dehydrogenase activity displayed a negative correlation with triazophos, bensulfuron-methyl
Sergio Ravasio et al.
Biochemistry, 41(25), 8120-8133 (2002-06-19)
The properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803 were determined by means of kinetic and spectroscopic approaches in comparison to those exhibited by the bacterial NADPH-dependent enzyme form. The ferredoxin-dependent enzyme was found to be similar to

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