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Iodonitrotetrazolium chloride

Used in colorimetric assays.

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


≥98% (TLC)




240 °C (dec.) (lit.)


H2O: 4 mg/mL


diagnostic assay manufacturing

storage temp.


SMILES string




InChI key


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Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.
Electron acceptor for the colorimetric assay of various dehydrogenases


250 mg in glass bottle
1, 5, 25 g in glass bottle


FlameExclamation mark

Signal Word


Hazard Statements

Hazard Classifications

Flam. Sol. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

4.1B - Flammable solid hazardous materials



Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Product I8377, Iodonitrotetrazolium chloride, is stated to have solubility in water, ethanol and ethylene glycol monomethylether.  What other solutions can be used?

    Conn's Biological Stains, 10th Edition (page 163) states P-Iodonitrotetrazolium Violet is also soluble in dimethylsulfoxide and dimethylsulfoxide-water mixtures.

  6. Can Product I8377, Iodonitrotetrazolium chloride, be used for the staining of cells?

    Yes, although due to the large crystal size of the formazan and lack of substantivity, histological application of I8377 has been limited.  In microbiology, the product was used by Lussenhop and Fogel (1993) for in situ microscopic observation of soil biota.. Gribbon and Barer (1995) used for the demonstration of viable but nonculturable bacteria.  Heidelberg (1997) used as a bacterial viability stain.

  7. Is Product I8377, Iodonitrotetrazolium chloride, lipophilic?

    Yes, I8377  is a lipophilic cation of moderate size.  The resulting formazan is more strongly liophilic and fat soluble.

  8. Is there a procedure for reducing Product I8377, Iodonitrotetrazolium chloride, by superoxide radicals?

    Yes.  See Biochem Biophys Res Commun. 1988 Feb 15;150(3):1294-301.

  9. Is there a reference for using Product I8377, Iodonitrotetrazolium chloride, to treat brain membrane preparations?

    Yes.  See Neuroscience Letters Volume 35, Issue 2, 21 February 1983, Pages 215-220

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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