K1637

Kanamycin sulfate from Streptomyces kanamyceticus

meets USP testing specifications, powder

• Synonym(s): Kanamycin A, Kanamycin sulfate salt
• Empirical Formula (Hill Notation): C₁₈H₃₆N₄O₁₁ · H₂O₄S
• CAS Number: 25389-94-0
• Molecular Weight: 582.58
• NACRES: NA.76
• PubChem Substance ID: 24896209
• UNSPSC Code: 51281654
• EC Number: 246-933-9
• MDL number: MFCD00070253

Properties

• InChI key: OOYGSFOGFJDDHP-KMCOLRRFSA-N
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
• SMILES string: OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• biological source: Streptomyces kanamyceticus
• agency: USP/NF, meets USP testing specifications
• form: powder
• potency: ≥750 μg per mg
• solubility: H₂O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)
• antibiotic activity spectrum: Gram-negative bacteria, Gram-positive bacteria, mycobacteria, mycoplasma
• application(s): pharmaceutical (small molecule)
• mode of action: protein synthesis | interferes
• Quality Level: 200

Description

General description

Kanamycin Sulfate is a broad spectrum aminoglycoside-antibiotic purified from the bacterium Streptomyces kanamyceticus. Kanamycin Sulfate is a selection agent for cells in culture media and acts by binding to the 30S subunit of the bacterial ribosome which inhibits protein synthesis in susceptible bacteria.

Application

Kanamycin sulfate is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL. Kanamycin sulfate from Streptomyces kanamyceticus has been used: in the research of cell type-specific expression of genes[1][2]

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Features and Benefits

• High quality antibiotic suitable for mulitple research applications
• meets USP testing specifications

Preparation Note

Tightly closed. Dry. Keep in a well -ventilated place. Keep locked up or in an area accessible

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Other Notes

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Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H360D
• Hazard Classifications: Repr. 1B
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• ppe: Eyeshields, Gloves, type N95 (US)
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501