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Sigma-Aldrich

Kanamycin sulfate from Streptomyces kanamyceticus

Animal Component-free

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Synonym(s):
Kanamycin A, Kanamycin sulfate salt
Empirical Formula (Hill Notation):
C18H36N4O11 · H2O4S
CAS Number:
Molecular Weight:
582.58
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.76

biological source

Streptomyces kanamyceticus

Quality Level

form

powder

potency

≥750 μg per mg (dry basis)

storage condition

(Tightly closed. Dry. Keep in a well -ventilated place. Keep locked up or in an area accessible )

impurities

≤5% Kanamycin B

color

white to off-white

solubility

H2O: 50 mg/mL, clear, colorless to faintly yellow

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
mycoplasma

mode of action

protein synthesis | interferes

storage temp.

15-25°C

SMILES string

OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1

Inchi Key

OOYGSFOGFJDDHP-KMCOLRRFSA-N

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1 of 4

This Item
K1637K1377PHR1487
vibrant-m

K1377

Kanamycin sulfate from Streptomyces kanamyceticus

Essential+ Grade
vibrant-m

PHR1487

Kanamycin Sulfate

-
mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

protein synthesis | interferes

mode of action

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

300

color

white to off-white

color

-

color

white to off-white

color

-

storage temp.

15-25°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

biological source

Streptomyces kanamyceticus

biological source

Streptomyces kanamyceticus

biological source

Streptomyces kanamyceticus

biological source

-

General description

Kanamycin Sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus.

application

Kanamycin sulfate is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL. Kanamycin sulfate from Streptomyces kanamyceticus has been used:
  • in the research of bacterial antibiotic resistance
  • in the research on the effect of different antibiotics on bud regeneration

Biochem/physiol Actions

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Features and Benefits

High quality antibiotic suitable for mulitple research applications

Preparation Note

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)


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