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MilliporeSigma

L1886

Light Green SF Yellowish

Dye content, ≥65%, certified by the Biological Stain Commission, powder

Synonym(s):

Acid Green 5

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About This Item

Linear Formula:
C37H34N2O9S3Na2
CAS Number:
5141-20-8
Molecular Weight:
792.85
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24896295
EC Number:
225-906-5
MDL number:
MFCD00012121
Beilstein/REAXYS Number:
5717828
Colour Index Number:
42095

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Product Name

Light Green SF Yellowish, certified by the BSC

InChI

1S/C37H36N2O9S3.2Na/c1-3-38(25-27-7-5-9-35(23-27)50(43,44)45)32-17-11-29(12-18-32)37(31-15-21-34(22-16-31)49(40,41)42)30-13-19-33(20-14-30)39(4-2)26-28-8-6-10-36(24-28)51(46,47)48;;/h5-24H,3-4,25-26H2,1-2H3,(H2-,40,41,42,43,44,45,46,47,48);;/q;2*+1/p-2

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)\C(=C3\C=CC(\C=C3)=[N+](/CC)Cc4cccc(c4)S([O-])(=O)=O)c5ccc(cc5)S([O-])(=O)=O

InChI key

DGOBMKYRQHEFGQ-UHFFFAOYSA-L

agency

certified by the BSC

composition

Dye content, ≥65%

technique(s)

titration: suitable

color

red to dark purple

mp

288 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

form

powder and chunks

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Show Differences

1 of 4

This Item
L5382B4014199583
Light Green SF Yellowish certified by the BSC

Sigma-Aldrich

L1886

Light Green SF Yellowish

Light Green SF Yellowish powder or crystals

Sigma-Aldrich

L5382

Light Green SF Yellowish

Brilliant Green certified by the BSC

Sigma-Aldrich

B4014

Brilliant Green

Lissamine™ Green B Dye content 60 %

Sigma-Aldrich

199583

Lissamine™ Green B

grade

certified by the Biological Stain Commission

grade

-

grade

certified by the Biological Stain Commission

grade

-

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

microbe id | staining: suitable

technique(s)

-

color

red to dark purple

color

dark red to purple

color

dark green

color

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder or crystals

form

powder

form

powder

solubility

H2O: 1 mg/mL

solubility

water: 10 mg/mL, clear

solubility

H2O: 1 mg/mL, blue

solubility

H2O: 0.1%

General description

Light Green SF Yellowish or Acid Green 5 is a triarylmethane dye that exists as disodium salt. It has the absorption value of 629 nm.[1]

Application

Certified for use as a cytological counterstain and as a substitute for aniline blue in Gomori′s one-step trichrome procedure.
Light Green SF Yellowish functions as an efficient histological stain for collagen. It is also used as colorant for foodstuff and beverages.[1]
Light Green SF Yellowish has been used to determine surface structures of trypsin treated tissue sections[2] and to stain buccal cells for slide scoring.[3]

Biochem/physiol Actions

Light Green SF Yellowish functions as an efficient histological stain for collagen. It is also used as colorant for foodstuff and beverages.[1]

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS6661
SHBS3497
0000391071
0000391071
SHBR5685

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Atomic force microscope investigation of the boundary-lubricant layer in articular cartilage.
Chan SM
Osteoarthritis and Cartilage, 18, 956-956 (2010)
Buccal micronucleus cytome biomarkers may be associated with Alzheimer's disease.
Thomas P
Mutagenesis, 22, 371-371 (2007)
Atomic force microscope investigation of the boundary-lubricant layer in articular cartilage
Chan SMT, et al.
Osteoarthritis and Cartilage, 18(7), 956-963 (2010)
The buccal cytome and micronucleus frequency is substantially altered in Down's syndrome and normal ageing compared to young healthy controls
Thomas P, et al
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 638, 37-47 (2008)
Decoloration treatment of a hazardous triarylmethane dye, Light Green SF (Yellowish) by waste material adsorbents
Mittal A, et al.
Journal of Colloid and Interface Science, 342(2), 518-527 (2010)
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