MilliporeSigma
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L5910

Millipore

Lysine medium

suitable for microbiology, NutriSelect® Plus

NACRES:
NA.85

sterility

non-sterile

Quality Level

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

final pH

5.0±0.2 (25 °C)

application(s)

food and beverages
microbiology

suitability

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Application

Used for isolation and enumeration of wild yeasts in pitching yeasts.

Components

Ingredients (g/L)
Dextrose, 44.50
Monopotassium phosphate, 1.78
Magnesium sulphate, 0.89
Calcium chloride, 0.178
Sodium chloride, 0.089
Adenine, 0.00178
DL-Methionine, 0.000891
L-Histidine, 0.000891
DL-Tryptophane, 0.000891
Boric acid, 0.0000089
Zinc sulphate, 0.0000356
Ammonium molybdate, 0.0000178
Manganese sulphate, 0.0000356
Ferrous sulphate, 0.0002225
L-Lysine, 1.00
Inositol, 0.02
Calcium pantothenate, 0.002
Aneurine, 0.0004
Pyridoxine, 0.0004
p-Aminobenzoic acid, 0.0002
Nicotinic acid, 0.0004
Riboflavin, 0.0002
Biotin, 0.000002
Folic acid, 0.000001
Agar, 17.80

Preparation Note

Suspend 6.6 g of Lysine Medium in 100 ml of distilled water containing 0.84 ml of potassium lactate solution ~60% (Cat. No. 60389). Boil to dissolve the medium completely. Cool to 50 °C. Adjust the pH to 5.0 with a 10% solution of Lactic Acid (Cat. No. 69778) and pour into sterile petri plates.NoteThe degree of contamination is expressed as the number of wild yeast cells per million cells of the original inoculum. The number of cells in the inoculum is important as a small number (100-1000) grow to a limited extent while 10′000 brewing yeast cells provide a direct measure of contaminant wild yeasts.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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