Lysine medium

suitable for microbiology, NutriSelect® Plus


Quality Level



product line




shelf life

limited shelf life, expiry date on the label


NutriSelect® Plus


microbe id | utilization test: suitable
microbiological culture: suitable
microbiology: suitable (microbial identification)

final pH

5.0±0.2 (25 °C)

Featured Industry

Food and Beverages


nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.


Used for isolation and enumeration of wild yeasts in pitching yeasts.


Ingredients (g/L)
Dextrose, 44.50
Monopotassium phosphate, 1.78
Magnesium sulphate, 0.89
Calcium chloride, 0.178
Sodium chloride, 0.089
Adenine, 0.00178
DL-Methionine, 0.000891
L-Histidine, 0.000891
DL-Tryptophane, 0.000891
Boric acid, 0.0000089
Zinc sulphate, 0.0000356
Ammonium molybdate, 0.0000178
Manganese sulphate, 0.0000356
Ferrous sulphate, 0.0002225
L-Lysine, 1.00
Inositol, 0.02
Calcium pantothenate, 0.002
Aneurine, 0.0004
Pyridoxine, 0.0004
p-Aminobenzoic acid, 0.0002
Nicotinic acid, 0.0004
Riboflavin, 0.0002
Biotin, 0.000002
Folic acid, 0.000001
Agar, 17.80

Preparation Note

Suspend 6.6 g of Lysine Medium in 100 ml of distilled water containing 0.84 ml of potassium lactate solution ~60% (Cat. No. 60389). Boil to dissolve the medium completely. Cool to 50 °C. Adjust the pH to 5.0 with a 10% solution of Lactic Acid (Cat. No. 69778) and pour into sterile petri plates.NoteThe degree of contamination is expressed as the number of wild yeast cells per million cells of the original inoculum. The number of cells in the inoculum is important as a small number (100-1000) grow to a limited extent while 10′000 brewing yeast cells provide a direct measure of contaminant wild yeasts.


We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Morris, E.O., et al.
Journal of the Institute of Brewing, 63(1), 34-34 (1957)
Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.
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