LIGHTOX25

Sigma-Aldrich

LightOx25

≥95% (HPLC)

Empirical Formula (Hill Notation):
C21H21NO2
CAS Number:
Molecular Weight:
319.40

assay

≥95% (HPLC)

form

powder

storage temp.

2-8°C

Related Categories

Application

Fluorescence microscopy, fluorescent retinoid binding assay, fluorescence spectroscopy.

Biochem/physiol Actions

Strong ligand for retinoic acid receptor proteins

Features and Benefits

LightOx25 is a synthetic analogue of all-trans-retinoic acid (ATRA), that exhibits solvatochromatic fluorescence emission when excited with UV or 405 nm light. The compound is a strong ligand for the retinoic acid receptor proteins (RARs) and related cellular retinoic acid binding proteins (CRABPI/II) and can, therefore, be used as a replacement for ATRA in cellular assays involving retinoid signaling processes. LightOx25 can be utilised to study retinoid behaviour using fluorescence microscopy, employed in a competitive fluorometric ligand-binding assay for direct determination of the strength of binding of non-fluorescent retinoids to a protein target, and as a fluorophore in fluorescence spectroscopy.

Other Notes

Chemical Equivalency 1ea=1mg

Legal Information

LightOx is a trademark of LightOx Ltd.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Articles
Fluorescent retinoic acid analogues for use in cellular imaging, protein-ligand binding assays, and flow cytometry to bypass traditional problems associated with fluorescent probes.
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