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Middlebrook OADC Growth Supplement

Enrichment supplement recommended for isolation and cultivation of Mycobacteria, suitable for microbiology

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Synonym(s):
Middlebrook Oleic Albumin Dextrose Catalase Growth Supplement, Oleic Albumin Dextrose Catalase
NACRES:
NA.85

Quality Level

sterility

sterile (Filtered and Aseptic Handled)

form

liquid

shelf life

limited shelf life, expiry date on the label

packaging

vial of 50 mL

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
veterinary

microbiology

storage temp.

2-8°C

suitability

mycobacteria

General description

Middlebrook OADC Growth Supplement is used with the Middlebrook Agar, that allows the isolation and cultivation of a wide variety of Mycobacteria. Middlebrook OADC Growth Supplement contains oleic acid, bovine albumin, sodium chloride, dextrose, and catalase. Oleic acid and other long chain fatty acids are essential for the metabolism of Mycobacteria. Some free fatty acids are toxic to Mycobacteria, but albumin binds to those fatty acids and prevents toxic action on Mycobacteria. Dextrose is the fermentable carbohydrate and an energy source whereas catalase destroys toxic peroxides that may be present in the medium.

Application

Middlebrook OADC growth supplement has been used as a supplement in 7H9 liquid medium to culture mycobacteria from clinical samples, environmental, food and beverages.

Components

Ingredients: (per vial)
Bovine Albumin Fraction V 2.50 g
Dextrose 1.00 g
Catalase 0.002 g
Oleic acid 0.025 g
Sodium chloride 0.425 g
Distilled water 50 mL

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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